高压处理种子对提高长春花中长春碱含量的作用

 在室温下对干燥的一年生矮种长春花种子进行了静高压氮气处理，压力分别为10、20、30 MPa，保压4 h。将各处理组与未经高压处理的对照组种子同时在相同的自然条件下进行播种培育。详细观察对比了各组植株的花形等性状，用高效液相色谱检测了生长旺盛期植株中长春碱和长春新碱的含量，另外还观察了剪枝后再生植株的生长性状。结果表明：种子经高压处理的各组长春花植株中的长春碱含量都有显著增加；长春新碱含量也有提高趋势。这种变化与花形改变和再生植株患病率的分布有一定程度的对应关系。初步认为植株体内长春碱含量的提高可能与高压导致的愈伤组织有关。

Effect of High Pressure Treating Catharanthus Roseus Seeds on Enhancing Content of Vinblastine in the Plant

Three groups of short annual Catharanthus roseus seeds were treated in high pressure nitrogen gas for 4 h at room temperature;the pressure was 10,20 and 30 MPa,respectively.Other untreated seeds were regarded as a control group(abbreviated to CP).All seeds were planted under same condition.The vegetal character of the Catharanthus roseus was observed in detail and contents of Vinblastine and Vincristine in the plants were detected by HPLC.Experimental results indicated that the contents of Vinblastine increased remarkably and Vincristine tended to increase in the plants of the pressure treated groups in comparison to CP.It is also noticed that distributions of variance of the flowers and ill aftergrowth plants are corresponding approximately to that of the contents of Vinblastine.Mechanism of the effects of pressure treatment was discussed qualitatively.