3-氨基苯硼酸为功能单体在壳聚糖上印迹牛血清白蛋白的研究

以壳聚糖为载体, 3-氨基苯硼酸为功能单体对牛血清白蛋白进行了分子印迹的研究, 并对吸附过程进行Langmuir等温吸附模型的数据处理. 结果表明, 印迹聚合物上形成了对于模板分子有较高的吸附容量和选择性的识别位点, Langmuir等温吸附平衡常数为49.5 mL/mg, 结合位点的最大表观结合量为16.3 mg/g, 证明了该印迹聚合物对于牛血红蛋白和溶菌酶这些非模板蛋白的吸附不具有选择性.

Study of Bovine Serum Albumin Imprinted Polymer Fabricated with 3-Aminophenylboronic Acid as Functional Monomer on Chitosan Particles

3-Aminophenylboronic acid was used as functional monomer,molecularly imprinted polymer of bovine serum albumin was fabricated on chitosan particles.The Langmuir adsorption model was undertaken to describe the equilibrium isotherms.Analysis of the model shows that many recognition sites of BSA were formed on the MIP with higher adsorption capacity and higher selectivity respect to the other two candidates.Langmuir adsorption equilibrium constant was 49.5 mL/mg and the theoretical maximum adsorption capacity was 16.3 mg/g.The imprinted polymers showed a better selectivity and capacity of the template than one of other competitive proteins.Therefore,this strategy and the functional monomer are promising in molecularly imprinting technology for separating and purifying each protein component in complex bio-samples.