Poly(N,N-dimethylacrylamide)-grafted polyacrylamide: a self-coating copolymer for sieving separation of native proteins by CE |
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Authors: | Zhang Jiyou Tran Nguyet Thuy Weber Jérémie Slim Cyrine Viovy Jean-Louis Taverna Myriam |
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Institution: | Groupe de Chimie Analytique de Paris Sud, Faculté de Pharmacie, 5 Rue JB Clement, F-92290 Chatenay-Malabry, France. |
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Abstract: | The potential of a series of newly synthesized poly(N,N-dimethylacrylamide) (PDMA) grafted polyacrylamide (PAM) copolymers (P(AM-PDMA)) as a replaceable separation medium for protein analysis was studied. A comparative study with and without copolymers was performed; the separation efficiency, analysis reproducibility and protein recovery proved that the P(AM-PDMA) copolymers were efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. Furthermore, the size-dependent retardation of native proteins in a representative P(AM-PDMA) copolymer was demonstrated by Ferguson analysis. The results showed that the P(AM-PDMA) copolymers combine the good coating property of PDMA and the sieving property of PAM and could be applied as a sieving matrix for the analysis of native proteins. |
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Keywords: | Native proteins Poly(dimethylacrylamide)‐grafted polyacrylamide copolymer Self‐coating Sieving |
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