Improved quantification limits in chiral capillary electrochromatography by peak compression effects |
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Authors: | Enlund Anna Maria Andersson Margareta E Hagman Gunnar |
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Institution: | Department of Chemistry, University of Basel, Spitalstrasse 51, 4004 Basel, Switzerland. |
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Abstract: | The peak compression effect has been applied to improve quantification limits in chiral capillary electrochromatography (CEC). A stationary phase based on the chiral selector vancomycin (Chirobiotic V) was used for separations of the enantiomers of mianserin. By adding solvents with a low dielectric constant, e.g. 2-propanol or tetrahydrofuran, to the sample solution, peak compression could be induced. The plate numbers for the minor enantiomer increased from approximately 100,000 to 1.4-1.6 million plates/m, when the composition of the mobile phase was adjusted so that the analyte eluted within either one of two system zones originating from the sample solution. A 10-fold improvement in the quantification limit for the minor enantiomer was obtained compared to elution under non-focused conditions. |
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Keywords: | Electrochromatography Enantiomer separation Peak compression Chiral stationary phases electrochromatography System zones Mianserin Vancomycin |
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