Action of α-cyclodextrin on phospholipid assemblies |
| |
Authors: | Valérie Bernat Catherine Ringard-Lefebvre Geneviève Le Bas Sylviane Lesieur |
| |
Institution: | (1) Laboratoire Physico-Chimie Pharmacotechnie Biopharmacie, UMR CNRS 8612, Université Paris-Sud, 5 rue Jean-Baptiste Clément, 92296 Chatenay-Malabry Cedex, France |
| |
Abstract: | Interactions of α-cyclodextrin (α-CD) with dimyristoylphosphatidylcholine (DMPC) and Egg phosphatidylcholine (Egg-PC) were studied (i) by analyzing surface
pressure-area isotherms and surface tension of phospholipid monolayers formed at the interface between air and α-CD aqueous solutions and (ii) by X-ray diffraction performed on fully hydrated α-CD/phospholipid binary mixtures. The cyclodextrin molecules strongly interact with the two-dimension phospholipid assembly.
Their addition into the aqueous sub-phase leads to the removal of part of the phospholipids from the air-water interface:
the higher the α-CD concentration, the higher the phospholipid depletion. This should preferentially involve interactions between cyclodextrin
and the phosphatidylcholine head group as α-CD is water-soluble and not surface-active. At the three-dimension level, the bilayer packing of the phospholipid lamellar
phase appears not affected by the presence of cyclodextrin as shown by X-ray scattering at small angles whereas wide-angle
diffraction patterns reveal the formation of a crystalline phase organized in a pseudo-hexagonal lattice usually characteristic
of α-CD dimers. These results point out that α-CD should interact with bilayer-forming phospholipid molecules but likely according to a process that would preserve intact
at least a part of the multilamellar assembly. |
| |
Keywords: | α -cyclodextrin Phosphatidylcholine Interaction Surface tension Surface pressure X-ray diffraction |
本文献已被 SpringerLink 等数据库收录! |