近红外光谱测定猪眼肌肌内脂肪中脂肪酸含量

为了快速无损测定猪眼肌肌内脂肪中脂肪酸含量,提出了近红外光谱技术检测的新方法。选用气相色谱法作为猪眼肌肌内脂肪中脂肪酸测定的标定方法,采用漫反射光纤探头采集由低密度聚乙烯塑料袋包装的猪眼肌样品的光谱，来测定眼肌内脂肪中脂肪酸的含量。利用光谱专用分析软件Unscrambler9.6, 对采集的光谱用平滑，二阶微分法进行预处理, 用偏最小二乘法(PLS)建立其定量检测模型。用于本实验的样本总数为82，将异常点除去后，将样品分为校正集和检验集。用校正集的样品来建立定标方程，再用它来预测检验集样品的值。结果表明，脂肪酸C14∶0，C15∶1，C16∶0，C16∶1，C18∶0，C18∶1，C18∶2，C18∶3，C20∶1，C20∶4，SFA，MUFA和PUFA校正模型的相关系数分别为0.57，0.76，0.71，0.77，0.62，0.81，0.86，0.91，0.85，0.91，0.67，0.81和0.95。可见, 用该方法测定肌内脂肪中脂肪酸有较高的测定精度，因此，应用近红外光谱对猪眼肌肌内脂肪中脂肪酸含量的快速无损检测具有可行性。

Detection of Fatty Acid Composition in Intramuscular Fat of Packed Pork Loin by Near Infrared Spectroscopy

Near infrared spectroscopy as a new method was proposed for rapid and non-destructive measurement of fatty acid composition in intramuscular fat of packed pork loin. Gas chromatography was used as a reference method for the spectral analysis of fatty acid composition. The fiber optic in interactance mode was adopted to measure the spectra of packed pork loins by low density polythene. The original spectra were pretreated by smoothing and 2nd derivative,and then PLS calibration model was builtby using softwa...