| 液相色谱-串联质谱检测贝类组织中5种脂溶性贝毒素 |
| |
| 引用本文： | 姚建华,谭志军,周德庆.液相色谱-串联质谱检测贝类组织中5种脂溶性贝毒素[J].分析化学,2010,38(12). |
| |
| 作者姓名： | 姚建华 谭志军 周德庆 |
| |
| 作者单位： | 中国水产科学研究院黄海水产研究所;上海海洋大学食品学院; |
| |
| 基金项目： | 国家863计划，国家科技支撑计划项目 |
| |
| 摘 要： | 建立了液相色谱-串联质谱分析贝类组织中米氏裸甲藻(GYM)贝毒素、螺环内酯毒素(SPX1)、大田软骨酸(OA)贝毒素、蛤毒素(PTX2)、原多甲藻酸(AZA1)贝毒素的方法.用甲醇-水(4: 1, V/V)溶液对贝类组织中GYM, SPX1, OA, PTX2和AZA1进行提取,MAX阴离子交换柱净化后,采用液相色谱分离,除OA以负离子选择反应监测外,GYM, SPX1, PTX2和AZA1以电喷雾离子源正离子选择反应监测模式进行质谱分析.5种脂溶性贝毒素GYM, SPX1, OA, PTX2和AZA1在各自相应浓度范围内线性良好,相关系数＞0.99.扇贝闭壳肌空白样品添加5种贝毒素的提取率均为78.6%～94.4%(n=6); 精密度(RSD)为6.8%～14.9%.贝类组织中5种贝毒素GYM, SPX1, OA, PTX2和AZA1的检出限分别为0.10, 0.21, 2.00, 0.32和0.04 μg/kg.
|
| 关 键 词： | 脂溶性贝毒素 贝类组织 液相色谱-串联质谱 |
Determination of Five Lipophilic Marine Toxins in Shellfish by Liquid Chromatography with Tandem Mass Spectrometry |
| |
| YAO Jian-Hua,TAN Zhi-Jun,ZHOU De-Qing.Determination of Five Lipophilic Marine Toxins in Shellfish by Liquid Chromatography with Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(12). |
| |
| Authors: | YAO Jian-Hua TAN Zhi-Jun ZHOU De-Qing |
| |
| Institution: | YAO Jian-Hua1,2,TAN Zhi-Jun1,ZHOU De-Qing 1 1(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071) 2(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306) |
| |
| Abstract: | A liquid chromatography-tandem mass spectrometric(LC-MS/MS) method for the determination of five lipophilic marine toxins including Gymnodimine(GYM),Spirolide-1(SPX1),Okadaic acid(OA) Pecenotoxin-2(PTX2) and Azaspiracid-1(AZA1) in shellfish was developed.After being extracted with methanol and water(80 /20,V/V),the extract was cleaned by solid phase extraction of MAX column,then determined by a reversed phase HPLC gradient program coupled with tandem mass spectrometry in selectedreaction-monitoring mode.GYM... |
| |
| Keywords: | Lipophilic marine toxins Shellfish Liquid chromatography-tandem mass spectrometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
