Improved Mass Multiplication of <Emphasis Type="Italic">Rhodiola crenulata</Emphasis> Shoots Using Temporary Immersion Bioreactor with Forced Ventilation |
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| Authors: | Yan Zhao Wei Sun Ying Wang Praveen K Saxena Chun-Zhao Liu |
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| Institution: | (1) National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China;(2) General Hospital of Air Force, Beijing, 100142, People’s Republic of China;(3) Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, People’s Republic of China;(4) Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada, N1G 2W1; |
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| Abstract: | A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at a temporary immersion cycle
of 3-min immersion every 300 min. Forced ventilation was employed in the temporary immersion bioreactor culture in order to
decrease the hyperhydration rate, improve shoot quality and enhance the multiplication ratio. The highest multiplication ratio
of 55.7 was obtained under a temporary immersion cycle of 3-min immersion every 180 min with the forced ventilation at an
air flow rate of 40 l/h, and the hyperhydration rate was reduced to 26.1%. Forced ventilation also improved the subsequent
elongation and rooting rate of these proliferated shoots, and the shoot cultures from the temporary immersion bioreactor formed
complete plantlets when subcultured onto a rooting medium containing 5 μmol/l indole-3-acetic acid. |
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