|
|||||
|
|
An efficient peptide ligation using azido-protected peptides via the thioester method |
|
| Authors: | Hidekazu Katayama Tsuyoshi Ohira |
| Institution: | a Department of Applied Biochemistry, Institute of Glycotechnology, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan b Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan |
| Abstract: | Azido-protected Fmoc-Lys-OH (Fmoc-Lys(N3)-OH) was synthesized from Fmoc-Lys-OH by the copper(II)-catalyzed diazo transfer method, and introduced to a peptide by the ordinary Fmoc-based solid-phase peptide synthesis. This azido peptide could be condensed with a peptide thioester by the Ag+-free thioester method without any significant side reactions. The azido group was easily reduced to an amino group by Zn powder after peptide condensation. |
| Keywords: | Azido peptide N-Alkyl cysteine Pigment dispersing hormones Thioester method |
| 本文献已被 ScienceDirect 等数据库收录！ | |