| 圆二色性测定蛋白质在超细粒子上吸附的构象变化 |
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| 引用本文： | 韩振为,钟成,王昌秀.圆二色性测定蛋白质在超细粒子上吸附的构象变化[J].分析测试学报,2004,23(2):27-30. |
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| 作者姓名： | 韩振为 钟成 王昌秀 |
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| 作者单位： | 天津大学,化工学院,天津,300072;天津大学精馏技术国家工程研究中心,天津,300072;天津华立达生物工程有限公司,天津,300241 |
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| 基金项目： | 国家自然科学基金资助项目 (29806010) |
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| 摘 要： | 研究了蛋白质在超细粒子固体表面吸附过程中构象的变化；选择的吸附剂粒径很小，可以直接应用圆二色性光谱测定蛋白质吸附层的二级结构变化；研究了pH、固体吸附表面在蛋白质吸附过程中对蛋白质构象的影响，得到了较好的实验数据，为进一步进行蛋白质吸附机理的理论研究提供了基础。
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| 关 键 词： | 蛋白质吸附 构象变化 圆二色性 |
| 文章编号： | 1004-4957(2004)02-0027-04 |
| 修稿时间： | 2003年4月29日 |
CD Studies on the Conformational Changes during Protein Adsorption on Ultrafine Particles |
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| HAN Zhen-wei,ZHONG Cheng,WANG Chang-xiu.CD Studies on the Conformational Changes during Protein Adsorption on Ultrafine Particles[J].Journal of Instrumental Analysis,2004,23(2):27-30. |
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| Authors: | HAN Zhen-wei ZHONG Cheng WANG Chang-xiu |
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| Institution: | HAN Zhen-wei1,2,ZHONG Cheng1,2,WANG Chang-xiu3 |
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| Abstract: | In order to study the conformational changes during protein adsorption on particulate surfaces, it is necessary to use ultrafine particles. The average diameters of the silica oxide and titanium oxide particles employed in our case are 15 and 20 nm, respectively. Since the intensity of light scattered by the ultrafine particles is negligible, direct estimation of conformational changes of adsorbed protein molecules from circular dichroism(CD) spectra is feasible. The effects of adsorption pH and the solid surfaces on protein conformational changes were studied. Data on protein conformational changes thus obtained can serve as a basis for further theoretical studies on the protein adsorption mechanism. |
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| Keywords: | Protein adsorption Conformational changes Circular dichroism |
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