Comparison of series 5 gas chromatography column performances from a variety of manufacturers for separation of chlorinated dibenzo-p-dioxins and dibenzofurans using high-resolution mass spectrometry

An extended study of seven fused silica capillary gas chromatographic (GC) columns has been conducted with regard to separation of international toxic equivalent factor (I-TEF) isomers (tetra- through octa-chlorinated at 2,3,7,8 positions) of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDDs/PCDFs) from closely co-eluted other isomers using high-resolution gas chromatography-high-resolution mass spectrometry (HRGC-HRMS). The data are explicated in mass chromatograms of Series 5 GC columns from a variety of manufacturers (Varian CP-Sil 8 CB LowBleed/MS, Phenomenex ZB-5UMS, Agilent HP-5MS, Restek Rtx-5MS, Supelco Equity-5, J&W Scientific DB-5 and DB-5MS), according to relative retention times, and 2,3,7,8-substituted isomer concentrations for each of the columns tested. Results showed differences between 5% phenyl methyl silicone and 5% silphenylene (Si-arylene) silicone polymer type GC stationary phases in separation of 2,3,7,8-substituted PCDDs/PCDFs from closely co-eluted isomers. The separation differences for Si-arylene type columns resulted in lower toxic equivalence (TEQ) values compared to the siloxane-based columns. Because of differences in product nomenclature and manufacturing practices by various manufacturers, incorrect assumptions and comparisons may be made regarding the interchangeability of these columns for PCCD/PCDF separations. The data presented are the most comprehensive to date and provide a valuable addition to operational criteria for the standard US Environmental Protection Agency methods 1613b and 8290.