Nylon and nylon blend nanotubes and nanorods |
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| Authors: | Valentina Simonetti Jason Y Park Nicholas J Panaro Larry J Kricka |
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| Institution: | (1) University of Bologna, Bologna, Italy;(2) Center for Biomedical Micro & Nanotechnology, Department of Pathology & Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA |
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| Abstract: | Nylon nanorods and nanotubes (200 nm diameter) were fabricated by the membrane wetting technique (solvent and melt wetting)
from a range of nylons (6; 6,6; 6,9; 6,10; 6,12; 11; 12, 6(3)T) and nylon blended with different dyes (Nylon Cast Blue, Nylon
6/6 Black) or with molybdenum disulfide (Nylon cast MDS). The 65-μm long nylon nanotubes and nanorods were characterized by
scanning electron microscopy. The nanoscale nylon 6,6 served as an effective high surface area alternative to a nylon membrane
as a solid support in a chemiluminescent assay for nylon-bound biotinylated nucleic acids based on streptavidin- alkaline
phosphatase and chemiluminescent detection of the bound alkaline phosphatase label with the dioxetane substrate, CDP-Star.
Layer-by-layer deposition of the cationic polymer (Sapphire-II™; Tropix) onto the nylon 6,6 nanostructures prior to UV-cross-linking
with biotinylated DNA resulted in further enhancement of binding and detection of biotinylated DNA. |
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| Keywords: | Nylon Nylon blend Membrane wetting Nanotube Nanorod Chemiluminescence Synthesis Nanobiotechnology |
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