土壤淋溶柱色谱中有机调节剂对保留的影响

 摘要：系统考察了55种非离子型化合物（包括11个氯代苯类、14个烷基苯类、22个多苯和多环芳烃类化合物、8个农药）在土壤淋溶柱色谱和较宽的淋洗剂（甲醇-水）组成范围内容量因子（k'）与甲醇体积分数（ψ）的关系。用自制加压装柱机将标准土壤干法装填成10mmi.d.×100mm的液相色谱柱，ψ=00.80的等度淋洗剂的流速1mL·min-1，柱温(25.0±0.1)℃，用UV检测器在线检测。实验表明，logk'=logkw+aψ+bψ2和logk'=logkw-Sψ两式均可以用来描述保留规律，但后者因其计算简

Influence of Organic Modifier on the Retention Behaviour in Soil Leaching Column Chromatography

The relationship between capacity factors (k') of 55 nonionic compounds and broad methanol volume percentage (psi) of methanol-water eluent in soil leaching column chromatography (SLCC) was systematically investigated. The compounds consist of 11 chlorobenzenes, 14 alkylbenzenes, 22 polyphenyls and polycyclic aromatic hydrocarbons, and 8 pesticides. Reference soil was dry-packed into a stainless steel chromatographic column (10 mm i.d. x 100 mm) by a homemade pressurizing device, and isocratic methanolwater mixture with psi from 0.0 to 0.80 eluated through the column at a flow-rate of 1 mL.min-1. The column was thermostated at (25.0 +/- 0.1) degree C, and chromatographic peak was monitored by an online ultraviolet detector. The results show that both equations, log k' = log k'w + a psi + b psi 2(1) and log k' = log k'w - S psi (2), well fit the retention values. Equation (2) can be used practically due to few experimental data needed and simpler in formula. Explanation is also given for the existence of the carbon (or chlorine) number rule for two classes of homologous series (i.e. methylbenzenes, n-alkylbenzenes) and weak-polar chlorobenzenes in the SLCC process. The slope and intercept of the rule are also well correlated, and both decreases linearly with increasing eluent psi value.