HPLC分离化学发光检测药物中的马钱子碱、士的宁和麻黄类生物碱

在KMnO₄-H⁺-还原剂体系中研究了马钱子碱、士的宁和麻黄类生物碱的化学发光(CL)行为,建立了其快速灵敏的流动注射化学发光分析方法,将此法与HPLC联用,实现了上述物质的选择性测定,该方法的检出限为1.0×10^-10g/mL马钱子碱、1.0×10^-8g/mL士的宁、1.0×10^-5g/mL麻黄碱和伪麻黄碱,测定的相对标准偏差小于3%,HPLC流动相的脉动及其中的溶解氧不影响测定的精密度。此法已用于一些药物中马钱子碱等生物碱的测定,回收率为94%～101%.

Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation

In this paper,the chemiluminescence reaction of brucine,strychnine and ephedrines in KMnO₄-H⁺-reductant system has been characterized with respect to their ability to act as a rapid and sensitive chemiluminescence determination system of the above alkaloids.This method has been connected with HPLC which fitted with a C₁₈ column for separation so as to determine these alkaloids in sample.The detection limits are 1.0×10^-10g/mLof brucine,1.0×10^-8g/mL of strychnine and 1.0×10^-5 g/mLof ephedrines, respectively.The relative standard deviation is less than 3% and recovery is 94%～101%.Since the dissolved oxygen did not affect the determination,this method provide advantages of wider linear range,high sensitivity and easy to operate.