Photocurable Nitric Oxide-Releasing Copolyester for the 3D Printing of Bioresorbable Vascular Stents |
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Authors: | Matheus F de Oliveira Laura C E da Silva Daniele M Catori Marcos V Lorevice Karen E A Galvão Ana L G Millás Marcelo G de Oliveira |
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Institution: | 1. Institute of Chemistry, University of Campinas, UNICAMP, Rua Josué de Castro, s/n, CP 6154, Campinas, SP, 13083-970 Brazil;2. 3D Biotechnology Solutions, 3DBS, Rua da Abolição, 1880, Campinas, SP, 13041-445 Brazil |
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Abstract: | The design of bioresorbable vascular stents (BVS) capable of releasing nitric oxide (NO) at the implant site may enable BVS to mimic the antiplatelet, antiproliferative, and pro-endothelial actions of NO, overcoming complications of BVS such as late thrombosis and restenosis. In this study, the fabrication of BVS composed of methacrylated poly(dodecanediol citrate-co-dodecanediol S-nitroso-mercaptosuccinate) (mP(DC-co-DMSNO)), a novel elastomeric, bioabsorbable, and photocurable copolyester, containing covalently bound S-nitrosothiol groups in the carbon backbone of the polymer, is reported. The mP(DC-co-DMSNO) stents are manufactured via photoinduced 3D printing and allow deployment via a self-expansion process from a balloon catheter. After deployment, hydration of the stents triggers the release of NO, which is maintained during the slow hydrolysis of the polymer. Real-time NO release measurements show that by varying the copolyester composition and the strut geometry of the mP(DC-co-DMSNO) stents, it is possible to modulate their NO release rate in the range of 30–52 pmol min−1 cm−2. Preliminary biological assays in cell culture show that endothelial cells adhere to the surface of the stents and that NO release favors their endothelization. Thus, mP(DC-co-DMSNO) may emerge as a new platform for the fabrication of advanced BVS. |
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Keywords: | 3D printing bioresorbable copolyester digital light processing nitric oxide vascular stents |
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