Dextran-coated CdSe quantum dots for the optical detection of monosaccharides by resonance light-scattering technique |
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Authors: | Shuang Hu Zhuo Bin Shang |
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Affiliation: | 1. School of Chemistry and Chemical Engineering, Shanxi University , Taiyuan, 030006, P.R. China;2. School of Pharmacy, Shanxi Medical University , Taiyuan, 030001, P.R. China;3. School of Chemistry and Chemical Engineering, Shanxi University , Taiyuan, 030006, P.R. China |
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Abstract: | A resonance light-scattering (RLS) detection method for saccharides was developed using dextran-coated CdSe quantum dots (dextran-CdSe-QDs) optical probes. The dextran-CdSe-QDs can be aggregated with concanavalin A (Con A), and the change in RLS intensity is used to monitor the extent of aggregation. The presence of glucose competitively binds with Con A, dissociating the Con A/dextran-CdSe-QDs complexes, affording the RLS intensity change and hence determining glucose concentrations in the range from a few to about 90 mM. Transmission electron microscopy was used to investigate the competitive interaction between glucose and dextran-CdSe-QDs with Con A. The competitive strategy could also be used to detect similar types of saccharides and the affinities of various monosaccharides for Con A increased in the order galactose?glucose < fructose < mannose. The proposed method was successfully applied to determine glucose in the human serum. A resonance light-scattering (RLS) detection method for saccharides was developed using dextran-coated CdSe quantum dots (dextran-CdSe-QDs) optical probes. The dextran-CdSe-QDs were coupled to concanavalin A (Con A) to facilitate the aggregation of nanoparticles. The presence of glucose competitively binds with Con A, dissociating the Con A/dextran-CdSe-QDs complexes affording the RLS intensity change and hence determining glucose in the range from a few millimolar to about 90 mM. The proposed method was applied to the determination of glucose in human serum samples with satisfactory results. |
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Keywords: | water-soluble quantum dots dextran concanavalin A monosaccharide resonance light scattering |
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