Production of lipase by a newly isolated <Emphasis Type="Italic">Bacillus coagulans</Emphasis> under solid-state fermentation using melon wastes |
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Authors: | Hüseyin Alkan Zübeyde Baysal Fikret Uyar Mehmet Dogru |
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Institution: | (1) Department of Chemistry, Faculty of Science-Art, Dicle University, Diyarbakir, Turkey;(2) Department of Biology, Faculty of Science-Art, Dicle University, Diyarbakir, Turkey |
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Abstract: | Summary An extracellular lipase was produced by Bacillus coagulans by solid-state fermentation. Solid waste from melon was used as the basic nutrient source and was supplemented with olive
oil. The highest lipase production (78,069 U/g) was achieved after 24h of cultivation with 1% olive oil enrichment. Enzyme
had an optimal activity at 37°C and pH 7.0, and sodium dodecyl sulfate increased lipase activity. NH 4NO3 increased enzyme production, whereas organic nitrogen had no effect. The effect of the type of carbon sources on lipolytic
enzyme production was also studied. The best results were obtained with starch and maltose (148,932 and 141,629 U/g, respectively),
whereas a rather low enzyme activity was found in cultures grown on glucose and galactose (approx 118,769 and 123,622 U/g,
respectively). Enzyme was inhibited with Mn+2 and Ni+2 by 68 and 74%, respectively. By contrast, Ca+2 enhanced enzyme production by 5%. |
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Keywords: | Solid-state fermentation Bacillus lipase production |
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