Glycine-conjugated porphyrin fluorescent probe with iRGD for live cell imaging |
| |
Authors: | Qian Zhang Xue Dong Kun-Peng Wang Ting-Ting Zhu Feng-Nan Sun Shu-Xian Meng Ya-Qing Feng |
| |
Institution: | a School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
b Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, China |
| |
Abstract: | A porphyrin modified by glycine has been synthesized and developed as a near-infrared(NIR) fluorescence probe to detect tumor.Porphyrins'longwavelength emission at ~650 nm can efficiently avoid the spectral crosstalk with Spontaneous fluorescence in the visible light region.A disulfide-based cyclic RGD peptide named iRGD c(CRGDKGPDC),a tumor homing peptide,harbors a cryptic C-end Rule (CendR)motif that is responsible for neuropilin-1(NRP-1)binding and for triggering extravasation and tumor penetration of the peptide to improve the imaging sensitivity and therapeutic efficacy.We used N-hydroxy succinimide as an activator to introduce the glycine methyl ester to detect tumor.We got a porphyrin modified by glycine.The affinity between probe and tumor cell entered GLC-82 cells(human glandular lung cancer cell line)can be observed by Confocal Microscope.The toxicity of probe has been identified by MTT Assay.The summary has been gotten that the porphyrins were nontoxic to GLC-82 cells and glycine modified porphyrin has a good affinity with GLC-82 cells under the iRGD function by our experiment. |
| |
Keywords: | Fluorescence probe iRGD Glycine Porphyrin Near-infrared |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《中国化学快报》浏览原始摘要信息 |
| 点击此处可从《中国化学快报》下载免费的PDF全文 |