快速老化模型小鼠海马蛋白质组学初步研究

应用双向凝胶电泳结合质谱鉴定, 分析比较6月龄和12月龄快速老化模型小鼠(Senescence-accele-rated mouse, SAM)的快速老化亚系SAM-prone/8(SAMP8)及抗快速老化亚系SAM-resistance/1(SAMR1)海马蛋白表达的差异, 从蛋白质水平初步探讨与老化相关的学习记忆功能障碍的发生机制. 结果表明, 与同龄SAMR1比较, 6月龄SAMP8海马中有15个蛋白点表达显著上调, 5个蛋白点表达显著下调; 12月龄SAMP8海马中有12个蛋白点表达显著上调, 2个蛋白点表达显著下调, 2个蛋白点只在SAMP8中有表达. 应用质谱分析结合数据库检索, 共鉴定了22种蛋白质. 6月龄和12月龄SAMP8与SAMR1海马中表达有明显变化的蛋白按功能可分为如下4类: (1) 能量代谢相关蛋白; (2) 线粒体功能相关蛋白; (3) 信号转导相关蛋白; (4) 其它蛋白. 研究结果表明, SAMP8和SAMR1海马蛋白表达存在明显差异, 其中一些蛋白与SAMP8随龄出现的学习记忆功能减退相关, 并可能为研究或发现促智药物作用的新蛋白靶标提供线索.

Preliminary Study on Hippocampal Proteomics of Senescence-accelerated Mouse

To investigate mechanisms of the deficits of learning and memory related with aging,the differentially expressed hippocampal proteins from 6-and 12-month-old SAM-prone/8(SAMP8)and age-matched SAMresistance/1(SAMR1)were analyzed and compared.In comparison with the same age SAMR1,15 proteins expressions in hippocampus of 6-month-old SAMP8 increased,5 proteins expressions decreased significantly;12 proteins expressions in hippocampus of 12-month-old SAMP8 increased,2 proteins expressions decreased significantly and 2 proteins only expressed in SAMP8 hippocampus;and 22 proteins with significant changes were identified by MALDI-TOF-MS and the results were searched in MASCOT database.These identified proteins could be devided into four categories according to their functions:(1) energy metabolism;(2) mitochondrion function;(3) signal transduction;(4) other proteins.The results show that there were significant differences in hippocampus protein expressions between SAMP8 and SAMR1,and some differentially expressed proteins were correlated with the deficits of learning and memory in SAMP8 with aging,and these proteins could provide clues for the study and discovery of new protein targets for improving intelligence drugs.