Fractionation of BSA and lysozyme using ultrafiltration: effect of pH and membrane pretreatment |
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| Institution: | 1. Faculty of Foundry Engineering, AGH University of Science and Technology, ul. Reymonta 23, 30-059, Kraków, Poland;2. Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060, Kraków, Poland;3. Faculty of Chemistry, Maria Curie-Skłodowska University, pl. Marii Curie-Skłodowskiej 3, 20-031, Lublin, Poland;1. Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, United States;2. Bristol-Myers Squibb, Global Manufacturing and Supply, Devens, MA 01434, United States;1. Leibniz-Institut für Polymerforschung Dresden, Department of Nanostructured Materials, Hohe Straße 6, 01069 Dresden, Germany;2. Technische Universität Dresden, Department of Chemistry, 01069 Dresden, Germany;3. Department of Materials Science & Engineering, Indian Institute of Technology (IIT) Delhi, New Delhi 110016, India;1. Department of Chemistry, Lorestan University, Khorramabad, Iran;2. Department of Chemistry, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran |
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| Abstract: | Selective transmission of a solute through membranes proves to be a challenge in ultrafiltration processes. This is because the transport of a solute through an ultrafiltration membrane does not depend on size alone, but on several other factors such as solute-solute and solute-membrane interactions. By manipulating physicochemical parameters and process variables (eg. pH, ionic strength, concentration of solute, etc.) and by membrane modification, it is possible to enhance the transmission of a particular solute and thus enhance fractionation of solutes. In this paper, the effect of pH on fractionation of BSA and lysozyme by ultrafiltration through 50 kDa MWCO (molecular weight cut off) polysulfone membrane has been examined. It was found that the selectivity of solute separation for dilute mixtures of BSA and lysozyme was very much pH dependent and varied from 3.3 at pH 5.2 to 220.0 at pH 8.8. However, at a higher feed concentration, the transmission of lysozyme through polysulfone membrane decreases quite dramatically resulting in lower throughput of product. An attempt has been made to enhance the transmission of lysozyme through the polysulfone ultrafiltration membrane by pretreating the surface of the membrane by adsorption of another protein, myoglobin. An increase in lysozyme transmission of up to 63% with respect to native membrane was observed. The stability of this pretreatment and its effect on permeate flux have been examined. The pretreated membrane was used to fractionate BSA/lysozyme mixtures. Even at higher feed concentration, enhanced fractionation with respect to native membrane was observed due to highly enhanced transmission of lysozyme through the pretreated membrane. |
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