基于COSM的三维显微图像复原算法研究

光学分层显微成像技术(COSM)不仅可用于去除非聚焦层杂散光的影响以及噪声的干扰,而且能复原样本的清晰三维像,将成为提取微流体的三维温度和速度场分布信息的有效工具.本文研究了基于已知点扩展函数的6种COSM成像算法,详细讨论了6种COSM成像算法的原理和实现,构造了三维样本图像和点扩展函数模型,并对复原结果进行了分析....     

A hybrid DSMC/Navier–Stokes frame to solve mixed rarefied/nonrarefied hypersonic flows over nano‐plate and micro‐cylinder

We extend a hybrid DSMC/Navier–Stokes (NS) approach to unify the DSMC and the NS simulators in one framework capable of solving the mixed non‐equilibrium and near‐equilibrium flow regions efficiently. Furthermore, we use a one‐way state‐based coupling (Dirichlet–Dirichlet boundary‐condition coupling) to transfer the required information from the continuum region to the rarefied one. The current hybrid DSMC–NS frame is applied to the hypersonic flows over nanoflat plate and microcylinder cases. The achieved solutions are compared with the pure DSMC and NS solutions. The results show that the current hybrid approach predicts the surface heat transfer rate and shear stress magnitudes very accurately. Some important conclusions can be drawn from this study. For example, although the shock wave region would be a non‐equilibrium region, it is not necessary to use a pure DSMC simulator to solve it entirely. This is important when the researchers wish to predict the surface properties such as velocity slip, temperature jump, wall heat flux rate, and friction drag magnitudes accurately. Our investigation showed that our hybrid solution time would be at least 40% (for the flat plate) and 35% (for the cylinder) of the time that must be spent by a pure DSMC solver to attain the same accuracy.Copyright © 2013 John Wiley & Sons, Ltd.     

微尺度型腔内液态金属流动规律模拟研究

修正了传统的Navier-Stoke方程,并利用修正后的方程与Fluent软件对微尺度型腔内Zn-Al合金微流动规律进行了数值模拟.模拟结果表明：液态金属在微尺度管道内的流动规律在管径为0.5mm时出现临界状态,管径越细,速度附面层相对厚度就越大.同时,微管流动存在着宏观流动中没有的“凸进效应”,且随着入口压力增大,流动前沿自由液面的凸进效应减弱.在流动前沿区域和上游区域之间出现负压力梯度区,且管径越细越明显. 关键词： 微管道 微流动 Zn-Al合金     

Investigation of the electrochemical oxidation products of zotepine and their fragmentation using on-line electrochemistry/electrospray ionization mass spectrometry

When zotepine, an antipsychotic drug, was electrochemically oxidized using electrospray ionization mass spectrometry (ESI-MS) coupled with a microflow electrolytic cell, [M + 16 + H]+ (m/z 348), [M-H]+ (m/z 330) and [M-14 + H]+ (m/z 318) were observed as electrochemical oxidation product ions (M represents the zotepine molecule). Although a major fragment ion that was derived from the dimethyl aminoethyl moiety was observed only at m/z 72 in the collision-induced dissociation (CID) spectrum of zotepine, new fragments such as m/z 315 and 286 ions could be generated in the CID spectrum by combining electrochemical oxidation and CID. Since these fragments were relatively specific with high ion strength, it was thought that they would be useful for developing a sensitive LC-MS/MS assay. The S-oxide and N-demethylated products were detected by electrolysis assuring that a portion of P450 metabolites of zotepine could be mimicked by the electrochemistry/electrospray ionization mass spectrometry (EC/ESI-MS) system.     

HPAM稀溶液在微圆管中流动特性的实验研究

实验研究了部分水解聚丙烯酰胺(HPAM)稀溶液(水溶液)在内径为10.1 ~325 \mu m石英微管中的高剪切速率(1 215 ~23 120 s ^{ -1} )流动. 结果表明, 聚合物溶液在管径小于100 \mu m微管中的流动具有明显的微尺度效应, 实测流速高于由同种溶液在常规管径圆管中的流动规律所预测的流速. 实测流速与预测流速之间的偏离程度与管径和剪切速率有关: 相同剪切速率下, 管径越小, 偏离越明显; 在小管径微管中, 偏离随剪切速率的增加而减小, 而当管径超过30.7 \mu m后, 偏离不再随剪切速率的变化而改变.      

Stability of two layers dielectric‐electrolyte microflow subjected to an alternating external electric field

The stability of the electroosmotic flow of the two‐phase system electrolyte‐dielectric with a free interface in the microchannel under an external electric field is examined theoretically. The mathematical model includes the Nernst–Plank equations for the ion concentrations. The linear stability of the 1D nonstationary solution with respect to the small, periodic perturbations along the channel, is studied. Two types of instability have been highlighted. The first is known as the long‐wave instability and is connected with the distortion of the free charge on the interface. In the long‐wave area, the results are in good agreement with the ones obtained theoretically and experimentally in the literature. The second type of instability is a short‐wave and mostly connected with the disturbance of the electrolyte conductivity. The short‐wave type of instability has not been found previously in the literature and constitutes the basis and the strength of the present work. It is revealed that with the increase of the external electric field frequency, the 1D flow is stabilized. The dependence of the flow on the other parameters of the system is qualitatively the same as for the constant electric field.     

Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems

To diversify metal-organic frameworks (MOFs), multi-component MOFs constructed from more than two kinds of bridging ligand have been actively investigated due to the high degree of design freedom afforded by the combination of multiple ligands. Predicting the synthesis conditions for such MOFs requires an understanding of the crystallization mechanism, which has so far remained elusive. In this context, microflow systems are efficient tools for capturing non-equilibrium states as they facilitate precise and efficient mixing with reaction times that correspond to the distance from the mixing point, thus enabling reliable control of non-equilibrium crystallization processes. Herein, we prepared coordination polymers with pillared-layer structures and observed the intermediates in the syntheses with an in-situ measurement system that combines microflow reaction with UV/Vis and X-ray absorption fine-structure spectroscopies, thereby enabling their rapid nucleation to be monitored. Based on the results, a three-step nonclassical nucleation mechanism involving two kinds of intermediate is proposed.     

单一微孔隙中油水相对渗透率特性研究

孔隙渗流是多孔介质渗流的微观基础。本文对孔隙中的两相流应用分相模型,结合达西渗流定律,提出了适用于油水两相的相对渗透率模型,得到相对渗透率与孔隙度无关、相对渗透率只是微孔隙中相态分布的几何尺度以及流体饱和度的函数等结论。设计了微流动实验装置,以去离子水和白油的乳化液为两相流体,在不同含油率下研究了微孔隙中油水两相的渗流特性,并与相应条件下的模型计算结果进行对比。结果表明,对于低渗透尺度等级的微孔隙,实验与计算结果吻合较好,对于高渗透尺度等级的微孔隙,实验与计算结果存在偏差。在某些条件下,油与水的相对渗透率之和存在大于1的情形。     

Adaptive finite elements with large aspect ratio for mass transport in electroosmosis and pressure‐driven microflows

A space–time adaptive method is presented for the numerical simulation of mass transport in electroosmotic and pressure‐driven microflows in two space dimensions. The method uses finite elements with large aspect ratio, which allows the electroosmotic flow and the mass transport to be solved accurately despite the presence of strong boundary layers. The unknowns are the external electric potential, the electrical double layer potential, the velocity field and the sample concentration. Continuous piecewise linear stabilized finite elements with large aspect ratio and the Crank–Nicolson scheme are used for the space and time discretization of the concentration equation. Numerical results are presented showing the efficiency of this approach, first in a straight channel, then in crossing and multiple T‐form configuration channels. Copyright © 2009 John Wiley & Sons, Ltd.     

On-Line Two-Dimension Liquid Chromatography for the Analysis of Ingredients in the Medicinal Preparation of Coptis Chinensis Franch

An on-line two-dimension microflow liquid chromatography was developed for better separation and analysis of the highly complex ingredients of medicinal preparation of traditional Chinese medicine Coptis Chinensis Franch. A two-valve switching system was utilized for two-dimension chromatography with strong cation exchange and reverse-phase capillary columns separation. The components were separated well by this system and yielded over 420 peaks. Under the optimal condition, 4 compounds were detected quantitatively. A good linear relationship was obtained from 0.2 µg mL^?1 to 24 µg mL^?1with detection limits (S/N = 3) ranging from 0.05 µg mL^?1 to 0.2 µg mL^?1for the compounds. We demonstrated that the method can be successfully applied to the analysis of a natural complex sample, with satisfactory results.