Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap‐Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules

High‐resolution mass spectrometry has been a powerful tool for the research of chemical constituents in traditional Chinese medicine (TCM) formulas. However, the chromatographic peaks were difficult to discriminate clearly in data collection or analysis because of the complexity and the greatly different content of the constituents in TCM formula, which increased the difficulty of identification. In this study, a high‐performance liquid chromatography coupled with linear ion trap‐Orbitrap mass spectrometry based strategy focused on the comprehensive identification of TCM formula constituents was developed. Identification was carried out from a high dose of medicinal materials to equivalent dose of formula. Meanwhile, combined with mass spectrometry data, chromatographic behaviors, reference standards and previous reports, the identification of constituents in Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules was described. 169 compounds were unambiguously or tentatively characterized, mainly including flavonoids, alkaloids, triterpenic acids, triterpene saponins, lactones, sesquiterpenoids and some other compounds. Among them, 11 compounds were unambiguously confirmed by comparing with reference standards. These results demonstrated that the method was effective and reliable for comprehensive identification of constituents of Xiang‐Sha‐Liu‐Jun‐Zi‐Jia‐Jian granules extracts and reveal the material basis of its therapeutic effects. This strategy might propose a research idea for the characterization of multi‐constituents in TCM formula.     

Chinese Physics C Subject Index to Vol.38(2014)

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Chinese Journal of Polymer Science Volume 32,Numbers 1-12,2014 CONTENTS

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CHINESE ANNALS OF MATHEMATICS SERIES B Volume 35 · 2014 TOTAL CONTENTS

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Author Index Vol.32,Nos.1-12,2014

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湘湖的自然地理及其兴废过程

萧山市湘湖是古代宁绍平原湖泊中众废残存的特例其前身西城湖早已埂废.北 宋 政和二年 (1 112年 ),因农业需要,在特殊的地理环境下又废田复湖.从北宋末至民国 间八百多年对湘湖主禁、主垦两派斗争激烈.1 9 4 9年以来,由于农田水利建设的发展,湘湖原有灌概职能减小,围垦扩大,成为目前一条狭长的河流型湖泊.但湘湖粘土层宜制砖瓦,挖泥造成深潭,出现了又一次还湖的情况,重新恢复的湘湖,为旅游业的发展创造了美 好的前景     

CONTENTS OF VOLUME 30

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SCIENCE CHINA Physics,Mechanics & Astronomy SUBJECT INDEX TO VOLUME 57(2014)

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SCIENCE CHINA Chemistry SUBJECT INDEX TO VOLUME 57(2014)

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关于割圆连比例解的项氏定理探析

借用项名达易率法、借率法和明安图多项式，本文分析证明关于割圆连比例解的项氏定理，澄清了易主经法的基本原理和方法特征，揭示了易率法与借径术、还原术三者之间的关系。