SE-HPLC/ICP-MS/ESI-MS联用技术用于富镉植物中镉的形态研究

用不同形态的镉培养液(氯化镉、硝酸镉、镉-EDTA、草酸镉和柠檬酸镉)对不同种类的蕨类植物(狗脊、舌厥、石松、瓦韦和石韦)进行培养。研究发现,镉在植物体不同部位的富集量是有差异的(富集量：根>茎>叶);对SEC-HPLC截取出的植物鳌合肽(PCs)研究表明,不同形态的镉能不同程度的诱发植物体中PCs的合成,从而PCs与Cd配位,降低镉的毒性,发现的配体主要为PC₃,iso-PC₃(βAla)和iso-PC₂(βAla)。文章还研究了不同形态镉胁迫下的蕨类植物各部位中镉的分布情况,并且对植物体中经镉诱导形成的PCs-Cd配合物的形态进行研究,进而对其解毒机理进行探讨。     

单扫描示波极谱法连续测定羊乳、黄芪、白鲜皮、蕨菜中铜和锌

研究了在HCl-KSCN底液中用单扫描示波极谱法连续测定羊乳、黄芪、白鲜皮、蕨菜中铜和锌含量的方法。在此底液中,铜和锌分别在-0．42和-1．03 V(vs．SCE)处产生一敏锐的二阶导数波,检出限分别为0．005和0．01 mg·L-1,线性范围分别为0．01～8．00和0．01-10．00 mg·L-1。应用此法测定羊乳、黄芪、白鲜皮、蕨菜中铜和锌含量,回收率在97．0％～101．7％。     

Subcellular distribution of rare earth elements and characterization of their binding species in a newly discovered hyperaccumulator Pronephrium simplex

Subcellular distribution of rare earth elements (REEs, including 14 lanthanides and yttrium) in a newly discovered REE hyperaccumulator, Pronephrium simplex (P. simplex), was determined by a chemical sequence extraction followed by ICP-MS analysis. Results showed that most REEs are associated with cell wall and proteins, and REEs concentration in the proteins, 2899.5 μg g⁻¹, is much higher than those in the cell wall; in the chloroplast of P. simplex, REEs distribute almost equally in chloroplast membrane and thylakoid, while most REEs in the thylakoid are binding with photosystem II (PS II); a new REE-binding peptide in the lamina of P. simplex, which can accumulate REEs up to 3000 μg g⁻¹ and has higher affinity with light REEs, was characterized, indicating that its molecular mass is 5073 Da, and may have β-sheet structure; isoelectrofocusing electrophoretic photograph indicated that it is acidic peptide with IP of 3.7. Such information should be useful for understanding of both the storage and physiological role of REEs in P. simplex and further studies on the phytoremediation of REEs contaminated environments.     

Brainin A,a novel flavanol from Brainea insignis

A novel flavanol bearing phenylpropionic acid moiety at C-4 through a C-C bonding,named brainin A(1),was isolated from the rhizomes of Brainea insignis.Its structure was elucidated on the basis of spectral data including 2D NMR and HR-ESI-MS.     

两种具有相同药效蕨类植物的红外光谱分析

利用傅里叶变换红外光谱仪测定了蕨和凤尾蕨的叶、茎和根的红外光谱图,分析比较了两个样品的叶、茎和根在红外光谱图上的差异程度,结果表明,尽管它们的药效均有清热利湿,消肿解毒等功效,但由于它们所含的化学成分不同和相对含量的差异,其红外光谱的吸收峰的位置和强度均有不同.通过对红外光谱图的主要特征峰的峰位、峰强的分析,推断出它们可能含有氨基酸、淀粉、少量鞣质等物质,由相应吸收峰的强度比推断出它们的叶均含有大量氨基酸、它们的根富含淀粉.此结果,对常用药用蕨类植物开发利用提供了科学的依据.