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A voltammetric enzyme-linked immunoassay based on a new system of ODA-H2O2-HRP has first been developed and used in the detection of HRP and labelled HRP. By this method, the enzyme-catalyzing reaction of H2O2 oxidizing odianisidine (ODA) couples the electrode-reduction reaction of the oxidizing product of odianisidine, which produces a sensitive polarographic wave at potential of -0.56V (SCE) in Britton-Robinson buffer solution. In using this polarographic wave, a detection limit to HRP is 3.7×10-12g/mL and a linear range 1.0×10-11-2.0×10-9g/mL. And the mechanisms of the coupling reaction and the process of electro-reduction in the ODA-H2O2-HRP voltammetric enzyme-linked immunoassay system have also been carefully studied. 相似文献
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葡萄糖氧化酶反应体系的动力学性质及在葡萄糖分析中的应用 总被引:1,自引:3,他引:1
本文运用紫外-可见光谱仪研究了葡萄糖氧化酶偶联辣根过氧化物酶-邻联茴香胺染料体系的催化动力学性质,通过控制酶和染料的含量,确定了反应呈假一级反应的葡萄糖浓度范围,在此反应体系中,葡萄糖浓度可以通过测定染料吸光度而获得,干扰小,回收率达97%~103%。 相似文献
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