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排序方式: 共有263条查询结果,搜索用时 62 毫秒
1.
2.
Capillary electrophoresis coupled to time of flight-mass spectrometry of therapeutic peptide hormones 总被引:2,自引:0,他引:2
We have established a method for separation and characterization of a series of peptide hormones of pharmaceutical interest and wide therapeutical use by capillary electrophoresis-electrospray-mass spectrometry (CE-ES-MS) using a sheath flow interface. Several parameters were systematically investigated, such as concentration of the electrolyte, organic solvent and sheath liquid composition, gas flow rates and capillary position. Moreover, limits of detection, linearity, repeatability and day-to-day reproducibility of the proposed method were studied in order to obtain the main quality parameters. 相似文献
3.
梯度洗脱测定植物源调节剂中内源激素方法探讨 总被引:8,自引:0,他引:8
建立了梯度洗脱高效液相色谱法同时测定内源激素赤霉素(GA3)、吲哚乙酸(3-IAA)、脱落酸(ABA)、细胞分裂素(6-BA)和α-萘乙酸(α-NAA)等5种植物内源激素的方法,采用Hypersil ODS C18柱和紫外检测器,以甲醇、乙腈和0.6%的乙酸为流动相梯度洗脱,分别在各组分的保留时间段设置其最佳检测波长,在12 min内可将上述5种内源激素的各组分分离完全,峰形良好,该方法的回收率可达到90%~96%,线性相关系数(r)大于0.998,检出限在0.02~0.3 μg/g之间.还探讨了各组分的最佳检测条件和梯度洗脱存在的问题及解决方法. 相似文献
4.
A method is described for the simultaneous quantitative determination of monoamines and related compounds from urine and brain tissue samples in the pg-range, using a GC/MF instrument LKB-2091 provided with a multiple ion detection (MID) system and capillary columns. The simultaneous detection of “fingerprints” with fourteen and more compounds during one single GC run was achieved after the intoduction of “time gates” for the detection of the different characteristic masses. Several modifications to the LKB equipment permitted exact reproducibility of retention times, which is essential for the use of “time gates”. The detection of “fingerprints” with these acidic, basic and alcoholic substances was achieved by the extraction of the dried biological samples with silylating agents. 相似文献
5.
Total internal reflection fluorescence (TIRF) microscopy is used to measure the dissociation kinetic rate of fluorescein-labeled epidermal growth factor from its specific receptors on the surface of intact but mildly fixed A431 human epidermoid cells in culture. Prior applications of TIRF microscopy have been limited to nonreceptor binding or to model membrane systems. The evanescent field excites fluorescence selectively at the surface of the cell proximal to the coverslip. Prismless epiillumination TIR is employed to avoid space limitations and is achieved by passing the excitation laser beam through a high (1.4)-aperture objective so that the light is incident at the glass/water interface beyond the critical angle. Long-term focus is maintained by a special feedback system. Of the possible effects that can influence the time course of the postbleach fluorescence recoveries—the EGF/receptor dissociation ratek
2, the bulk solution diffusion rate of EGF, and the cell surface motion of the receptors—we infer that the dissociation ratek
2 dominates. Several fitting schemes are compared and indicate the presence of a multiplicity of values fork
2, ranging from about 0.05 to 0.004 s–1, with an average value of about 0.012 s–1. These results compare well with values previously obtained by radiolabel/washing techniques. The significance of the results in terms of kinetic models and the advantages of the TIRF technique for these sorts of measurements are discussed. 相似文献
6.
Steroid hormones are a diverse group of natural and synthetic compounds. Their wide use in human and veterinary medicine results in their continual introduction into the environment. In recent years, environmental concern over steroids that act as endocrine disruptors has increased because of their adverse effects on organisms or their progeny. Moreover, as these compounds are not totally removed from sewage in wastewater treatment plants, they can reach the aquatic environment and persist due to their physicochemical characteristics.For this reason, a major trend in analytical chemistry is the development of rapid and efficient procedures for the extraction, determination and quantification of steroid hormones in environmental samples. Over the past few decades, the significant expansion of liquid chromatography technology utilizing mass spectrometry detection has led to applications with increased selectivity and sensitivity. Optimized extraction and microextraction techniques combined to these liquid chromatography techniques have lowered detection and quantification limits to the ng L−1–μg L−1 range, which is the concentration of steroid hormones in liquid, solid and biota samples.In this paper, the state-of-the-art techniques for the analysis of steroid hormones focused mainly in based liquid chromatography methods in liquid and aquatic solid and biota samples are reviewed. Handling, storage, extraction and detection methodologies are reviewed and compared for all families of steroid hormones. 相似文献
7.
Filipa M.B. L William L. Ledger Jane W. Davidson David M. Howard Georgina L. Jones 《Journal of voice》2007,21(6):754-761
The success of professional operatic singers depends upon the quality of their vocal mechanism. This is known to be sensitive to changes in the endocrine environment. Despite a widespread belief among singers that vocal quality changes according to the stage of the menstrual cycle, this has received little attention. In particular, the possibility that use of the contraceptive pill might stabilize vocal quality by "dampening" hormonal fluctuations has not previously been studied systematically. Here, we show that drospirenone containing oral contraceptive pill (Yasmin, Schering AG, West Sussex, UK) with antiandrogenic and antimineralocorticoid properties demonstrates a significant reduction in the irregularity of the pattern of vibration of the vocal folds during the performance of highly trained classical singers. This study constitutes the first double blind, randomized placebo controlled trial to assess the effects of the contraceptive pill on the patterns of vibration of the vocal folds during the performance of Western classical singing repertoire. 相似文献
8.
9.
Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction 总被引:17,自引:0,他引:17
A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC–ELISA (RIA) or HPLC–MS. 相似文献
10.
Tijen Tanyalcin Diler Aslan Yusuf Kurtulmus Nalan Gökalp Kamil Kumanlioğlu 《Accreditation and quality assurance》2000,5(9):383-387
In this study the reference intervals for folate and vitamin B12 were estimated according to the National Committee for Clinical Laboratory Standards Approved Guideline C28-A and International
Federation of Clinical Chemistry recommendations. The study included 155 women and 124 men between ages 18–40. The health
status was confirmed by history, physical examination and a questionnaire. The central 95% reference intervals of serum folate
and vitamin B12 for women, determined non-parametrically, were found to be 3.9–18.1 ng/ml and 101–666.7 pg/ml, respectively.
The reference values of serum folate and vitamin B12 for men were also found to be 2,5–17.6 ng/ml and 100–699.57 pg/ml, respectively.
We did not observe subclass differences between females and males.
Received: 15 April 2000 · Accepted: 15 April 2000 相似文献