排序方式: 共有33条查询结果,搜索用时 109 毫秒
1.
以催化极谱法测定了54例白血病患者血清硒含量。结果表明,急淋、急粒患者血清硒含量均低于正常对照组,慢粒及急、慢粒经治疗缓解者血清硒含量与正常对照组间均无显著性差异,提示低硒状态只存在于急性患者,且随病情缓解后血硒水平趋于正常,慢粒与血硒含量没有明显的相关关系。 相似文献
2.
5-Aminolaevulinic acid-mediated photodynamic therapy in multidrug resistant leukemia cells 总被引:5,自引:0,他引:5
Li W Zhang WJ Ohnishi K Yamada I Ohno R Hashimoto K 《Journal of photochemistry and photobiology. B, Biology》2001,60(2-3):79-86
To verify if photodynamic therapy (PDT) could overcome multidrug resistance (MDR) when it it applied to eradicate minimal residual disease in patients with leukemia, we investigated the fluorescence kinetics of 5-aminolaevulinic acid (ALA)-induced protoporphyrin IX (PpIX) and the effect of subsequent photodynamic therapy on MDR leukemia cells, which express P-glycoprotein (P-gp), as well as on their parent cells. Evaluation of PpIX accumulation by flow cytometry showed that PpIX accumulated at higher levels in mdr-1 gene-transduced MDR cells (NB4/MDR) and at lower levels in doxorubicin-induced MDR cells (NOMO-1/ADR) than in their parent cells. A P-gp inhibitor could not increase PpIX accumulation. Measurement of extracellular PpIX concentration by fluorescence spectrometry showed that P-gp did not mediate the fluorescence kinetics of ALA-induced PpIX production. Assessment of ferrochelatase activity using high-performance liquid chromatography indicated that PpIX accumulation in drug-induced MDR cells was probably regulated by this enzyme. Assessment of phototoxicity of PDT using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that PDT was effective in NB4, NB4/MDR, NOMO-1 and NOMO-1/ADR cells, which accumulated high levels of PpIX, but not effective in K562 and K562/ADR cell lines, which accumulated relatively low levels of PpIX. These findings demonstrate that P-gp does not mediate the ALA-fluorescence kinetics, and multidrug resistant leukemia cells do not have cross-resistance to ALA-PDT. 相似文献
3.
Sreekanth Thota Mohammad Imran Manasa Udugula Rajeshwar Yerra Subhas S.Karki Jan Balzarini Erik De Clercq 《中国化学快报》2012,23(4):466-469
The synthesis and spectroscopic characterization of ruthenium complexes(R-l to R-8) of the type[Ru(A)2(B)],(where A = 1,10-phenanthroline/2,2′-bipyridine and B = 3.4.5-tri-OCH3DPC,4-CH3-DPC,4-N-(CH3)2-DPC,4-NO2-DPC arc described. These ligands form bidentate octahedral ruthenium complexes.The title complexes were subjected to in vitro cytotoxic activity measurements against the human cancer T-lymphocyte cell lines MTT assay.In vitro evaluation of these ruthenium complexes revealed cytotoxic activity from 0.24 to 1.4μg/mL against CEM,0.44 to 1.9μg/mL against Molt4/C8.0.28 to 1.5μg/mL against L1210,0.24 to 0.98μg/mL against HL60,and 0.25 to 1.2μg/mL against BEL7402,depending the nature of the compound. 相似文献
4.
A Study on Uptake and Localization of Merocyanine 540 (MC540) in Murine Myeloid Leukemia Cells by Confocal Laser Scanning Microscopy (CLSM) 总被引:1,自引:0,他引:1
J. Y. Chen- N. H. Cheung- S. C. Chen- M. C. Fung- W. N. Leung- N. K. Mak- 《Chinese Journal of Lasers》2000,9(3):226-232
1 Introduction Merocyanine540(MC540),anamphipathicdyethatpreferentiallybindstoleukemiacell,envelopedvirusandcertainvirusinfectedcells[1~3].MC540mediatesthephotodynamickillingofleukemiacells,whereasnormalbonemarrowandbloodcellsarelargelyspared.Ithasbeenusedpre… 相似文献
5.
StructuralandAntileukemicStudiesofIndirubinDerivativesTIANFa-an,LIChun-minandLIDai-yu(DeparrmentofChmistry,DeparsmentofMedici... 相似文献
6.
LI Ming-tang JIANG Yong WANG Shu-mei LI Yong-ming WANG Fang HOU Xia YANG Hong MA Tong-hui 《高等学校化学研究》2006,22(2):145-149
Introduction Theleukemiainhibitoryfactor(LIF)isamulti functionalcytokinebelongingtotheinterleukin6(IL6)family[1].Itwasfirstfoundasamyeloidleukemic cellproliferationsuppressoranddifferentiationinducer cytokine[2].ThenaturalformofLIFisa38to67kDa glycosylate… 相似文献
7.
Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification 总被引:5,自引:0,他引:5
Cui JW Wang J He K Jin BF Wang HX Li W Kang LH Hu MR Li HY Yu M Shen BF Wang GJ Zhang XM 《Electrophoresis》2005,26(1):268-279
Two-dimensional electrophoresis (2-DE) was used to profile the proteins of leukemic cells from 61 cases of akute leukemia (AL) characterized by the French-American-British (FAB) classification. The differentially expressed protein spots were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray ionization-tandem MS (ESI-MS/MS). The distinct protein profiles (DPPs) of AL FAB subtypes were explored successfully, including acute myeloid leukemia (AML), its subtypes (M2, M3, and M5), and acute lymphoid leukemia (ALL), which were homogeneous within different samples of the same subgroup but clearly differed from all other subgroups. We also found a group of proteins differentially expressed between AL cells and normal white blood cells. Among the DPPs of AL subtypes, some proteins have been reported, but most of them were first reported here to mark AML differentiation and to discriminate AML from ALL. These data show that 2-DE protein profiling could be used as an analytical tool for facilitating molecular definition of human AL classification and understanding the mechanism of leukemogensis, and the extension of the present analysis to the currently less well-defined AL will identify additional subgroups and may promote the identification of new targets for specific treatment approaches. 相似文献
8.
硒抑制体外白血病细胞的生长和增殖.并能诱导部分白血病细胞分化成熟。但其药理作用因硒制剂的不同而异.硒代二半胱氨酸抑制V937和K562血病细胞的生长和增殖,半数抑制浓度为3.0μmol/L,经30μmol/L硒代二半胱氨酸作用3天后,U937胞吞噬率以5%上升至14%,K562细胞内血红蛋白含量由0.20增至0.40μg/10^6细胞,说明硒代二半胱氨酸能诱导部分白血病细胞分化成熟。 相似文献
9.
Afang Liu Meng Qing Yuliang Pan Ying Peng Manli Guo Yan Huang Zhou Nie Shouzhuo Yao 《Electroanalysis》2013,25(7):1780-1786
A novel electrochemiluminescence (ECL) method for label‐free detection of cancer cells was proposed for the first time by capturing negatively charged Jurkat cells onto Ru(bpy′)${{{2+\hfill \atop 3\hfill}}}$ ‐immobilized indium tin oxide (ITO) electrode via electrostatic interaction. The ECL sensor exhibited excellent sensitivity, good stability and a linear response to Jurkat cells in the concentration range from 1×103 to 2×105 cells/mL, with a detection limit of 730 cells/mL. Furthermore, the method was successfully applied in the study of cell growth and cell apoptosis, which was supported by fluorescent images analysis. The proposed protocol is simple, rapid, inexpensive and universally targetable for tumors, offering a novel platform for the development of an ECL biosensor for cell detection. 相似文献
10.
In this study, we have fabricated the functionalized nickel nanoparticles and investigated their effects on cellular uptake of quercetin in leukemia K562 cancer cells by using electrochemical assay. The results indicate that nickel nanoparticles could efficiently enhance the quercetin uptake and increase the intracellular accumulation in cancer cells, implying the great potential of functionalized nickel nanoparticles in target cancer therapy. 相似文献