Die quantitative Verfolgung des Sulfurierungsprozesses

Ohne Zusammenfassung     

On the Hausdorff dimension of generalized Besicovitch-Eggleston sets of d-tuples of numbers

We give a systematic and detailed account of the Hausdorff dimensions of sets of d-tuples of numbers defined in terms of the asymptotic behaviour of the frequencies of strings of digits in their N-adic expansion.     

PROTEIN STRUCI'URE MODELLING OF THE BACTERIAL LIGHT-HARVESTING COMPLEX

Abstract Protein structure modelling offers a method of obtaining 3-dimensional information that can be tested and used to plan mutagenesis experiments when a crystallographically determined structure is not available. At its simplest a model may consist of little more than a secondary structure prediction coupled with a determination of the likely regions of transmembrane/membrane surface/globular configuration. These methods can yield an interesting topology map of the protein, which places the residues in their likely positions with respect to, for example, the membrane interface. If it is a member of a large family of related proteins then aligned protein sequences can be used to predict the residues that have an important function as these. will be largely conserved in the alignments. Using all these methods a model can be constructed (using for example, the Nicholson Molecular Modelling Kit) to visualize the proposed structure in three dimensions following the premise of good design, that is, avoiding obvious steric clashes, packing of helices in a realistic manner, observing the correct H-bond lengths, etc . In this latter exercise the review of Chothia ( Annu. Rev. Biochem . 53 , 537–572, 1984) of the principles of protein structure is particularly helpful as it clearly sets out how proteins pack and their preferred configuration. There is a wealth of information about individual amino acid conformational preferences and observed frequencies of occurrence in known protein structures, which can help decide how the residues in the model can be oriented.
In this article we have collated the various protein models of the bacterial light-harvesting complexes and present our own model, which is a synthesis of the available biophysical data and theoretical predictions, and show its performance in explaining recent results of site-directed mutants of the LHI and LH2 light-harvesting complexes of Rhodobacter sphaeroides .     

A high precision spectrophotometric method for on-line shipboard seawater pH measurements: the automated marine pH sensor (AMpS)

Measurement strategies for understanding the oceanic CO(2) (carbon dioxide) system are moving towards in situ and ship of opportunity sampling techniques. Automated instrumentation with high accuracy and sampling frequencies will enable a greater understanding of the fluxes of marine carbon and lead to a more reliable constrain on the calculated uptake of anthropogenic CO(2) by the oceans. This paper describes the automated marine pH sensor (AMpS); new instrumentation and methodology for the determination of seawater pH using dual spectrophotometric measurements of sulfonephthalein indicator in a semi-continuous seawater stream. The pH values measured during a recent study in the Weddell Sea are used to illustrate the excellent properties of the AMpS. The method has an on-line precision of better than 0.001 pH units and an estimated accuracy of better than 0.004 pH units. The instrument is compact, portable and has a measurement frequency of 20 samples per hour. The instrument is ideally suitable for operation on ships of opportunity.