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以0, 0.05, 0.1, 0.25, 0.5 Gy 12C6+ 离子全身预辐照昆明小鼠, 间隔4 h后再对小鼠进行4 Gy全身辐射。 辐照后12 h用流式细胞仪检测小鼠胸腺脾脏细胞在各细胞周期时相的百分率, 同时用单细胞电泳检测受辐射小鼠胸腺脾脏细胞DNA损伤程度。 结果显示, 相对于大剂量预照射组, 各低剂量预照射组胸腺细胞S期细胞百分率显著减少; 脾脏细胞G0/G1期细胞百分率明显减少; 同时胸腺脾脏细胞的拖尾率及拖尾长度明显减少, 以0.1 Gy预辐照效果最为明显。 这些结果表明, 低剂量预辐射处理可以减弱胸腺细胞的S期阻滞及脾脏细胞的G1期阻滞, 并明显减轻胸腺脾脏细胞的DNA损伤程度。  相似文献   
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辐射免疫学研究进展   总被引:4,自引:0,他引:4  
近年来辐射免疫学作为一门新兴学科进展迅速,特别是在低剂量辐射兴奋效应研究方面有较大发展,同时重离子辐射生物效应的研究在治疗肿瘤方面也有了明显进步。综述了国内、外辐射免疫学的现况,其目的是寻找出一种综合疗法,从而达到既可以较好的治疗肿瘤,又对病人损伤较小的目的。Radiation immunology as a new subject has made a great progress in recent years, especially in the radiation hormesis. At the same time, the research of radiobiological effect on heavy ions has played an important role in the cancer therapy, especially on the radiation immunology of heavy ions in the outer space. In this review, we summaried the status and development of radiation-immunology, and try to find out some better ways which can increase efficient killing on tumours, but reduce the damages on normal tissues.  相似文献   
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用腺病毒重组体(AdCMV p53/GFP)转染经0.5, 1.0和2.0 Gy γ射线辐射处理的前列腺癌细胞[PC 3( nullp53)], 用克隆形成法检测细胞增殖能力, 用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。 结果提示, 辐射诱导使腺病毒重组体转染PC 3细胞提高7%—39%。 辐射联合 AdCMV p53 转染组p53表达水平提高18.5%—35.4%。 与单纯 AdCMV p53 转染组和单纯辐射组相比, 辐射联合 AdCMV-p53 转染组细胞存活率分别降低25%—64%和22%—65%。 To determine whether low dose pre irradiation could enhance adenovirus mediated p53 transfer and expression in human prostate adenocarcinoma, the PC 3 cells were pre exposed to γ rays, and then infected with replication deficient adenovirus recombinant vectors, containing human wild type p53 (AdCMV p53) or green fluorescent protein gene (AdCMV GFP) respectively (γ ray irradiation + AdCMV p53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMV GFP infection groups were 7%—39% more than those in AdCMV GFP infection groups. The p53 levels in the γ irradiation + AdCMV p53 infection groups were 18.5%—35.4% more than those in AdCMV p53 infection groups (p<0.05),suggesting that low dose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC 3 cells. The survival fractions for the γ irradiation + AdCMV p53 infection groups were 25%—64%, 22%—65% less than those for AdCMV p53 infection, or γ irradiation groups, respectively (p<0.05).  相似文献   
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探讨姜黄素(Curcumin,简称Cur)对重离子辐射损伤小鼠睾丸组织的防护作用。小鼠灌胃不同剂量的Cur后给予4 Gy剂量~(12)C~(6+)离子束全身单次照射。24 h后对小鼠睾丸组织形态学变化进行观察,并测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性。结果显示:中、高浓度Cur预处理组对小鼠睾丸组织的形态具有较好的保护作用;低、中浓度Cur预处理组MDA含量显著降低(P0.05);与单纯照射组相比,低浓度Cur预处理组SOD活性水平和中浓度Cur预处理组CA了活性水平显著提高(P0.05)。结果表明:Cur对重离子全身辐射小鼠的抗氧化系统有一定的激活效应,对辐射损伤有一定的防护作用,其机制可能与Cur清除自由基,保护脂质和蛋白质有关。  相似文献   
5.
重点综述了Siah, HIF, NF-κB和DNA-PK蛋白与肿瘤细胞辐射敏感性关系的最新研究进展。 总结了中国科学院重离子束辐射生物医学重点实验室近几年来在BRCA1等肿瘤细胞辐射敏感性相关蛋白方面的研究工作。 简介了HLET C离子束治疗肿瘤的优点。 展望了此实验室借助兰州重离子研究装置(HIRFL)提供的C离子束研究肿瘤细胞辐射敏感性相关蛋白的目标和方向。 The research progress of tumor cells radiosensitivity associated protein Siah, HIF,NF-κB and DNA-PK are summarized and reviewed. The recent works of our laboratory on tumor cells radiosensitivity associated proteins such as BRCA1 are demonstrated. In the present review, we focused on discussions about the advantages of heavy ion therapy and its possible application in the research of radiosensitivity associated proteins. At the end of this review, we highlighted the future trend and potential targets in the study of tumor cells radiosensitivity associated proteins.  相似文献   
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为了评估低剂量X射线连续辐射对BALB/c小鼠健康机体免疫系统的影响, 实验采用X射线全身连续照射BALB/c小鼠, 照射第一天剂量为0.07 Gy, 剂量率0.2 Gy/min, 之后每天照射0.08 Gy, 共照射12 d, 累积剂量1.03 Gy, 照射后24和48 h取血、胸腺和脾脏。 流式细胞仪检测免疫细胞周期和凋亡的变化, 胸腺和脾脏指数用重量法获取。 实验结果表明, 小鼠胸腺细胞的周期在照射后24 h被阻滞在G2/M期; 外周血淋巴和胸腺细胞周期48 h被阻滞在 G0/G1期, 细胞凋亡比例在照射后两个时间点都显著增加; 脾脏淋巴细胞周期24 h被阻滞在 G0/G1期, 48 h被阻滞在 S期, 细胞凋亡比例在24和48 h显著减少; 脾脏指数在照射后48 h显著减少。 故低剂量X射线连续全身照射BALB/c小鼠可激活免疫细胞不同的周期监测点, 引起免疫细胞凋亡比例发生变化, 造成一定的辐射损伤, 且这种影响随着免疫器官的不同而不同。 For estimating the effect of low doses X ray continual irradiation to immunity system of mouse, BALB/c mice were continually irradiated to 1.03 Gy by X rays at a dose rate of 0.2 Gy/min in 13 d. At 24 or 48 h after irradiation, the immunocyte cycle and apoptosis were determined by flow cytometry, and the thymus and spleen weights were measured too. The results showed that the cycle of thymocyte were arrested in G2/M at 24 h, the number of peripheral blood lymphocytes and thymocytes in G0/G1 phase at 48 h was up and the percentage of apoptosis had a significance increase in both of time points; the cycle of spleen lymphocytes was delayed in G0/G1 at 24 h, in S phase at 48 h, the apoptosis had a significance decrase at 24 and 48 h; spleen index declined significantly at 48 h. The results suggested that low doses continual X ray whole body irradiation could activate different cell cycle checkpoints, and result in some changes of apoptosis and some damages to immunocytes. The continual X ray irradiation affects the organs differently, it might provide experiment basis for radioprotection.  相似文献   
7.
综述了蛋白质组学的主要方法和技术——双向凝胶电泳、生物质谱技术、蛋白质芯片技术,及其在肿瘤标志物的筛选和鉴定、肿瘤治疗效果的评价及肿瘤发生机制研究等方面的应用状况,并对蛋白质组学在重离子治疗中的应用进行了展望。With the accomplishment of the human genome project, proteomics becomes a new snbject on the buildup of the whole proteins and their dynamic changes in cell emerges. Cancer is a kind of complex disease involved in multi-genes and proteins. Heavy ion therapy is all arising and potential radiation treatment nowadays. This paper reviews on the main methods and technology in proteomics-- two-dimensional gel electrophoresis (2-DE),biological mass spectrometry, protein biochips, bioinformatics and its application on identification of cancer biomarkers, evaluation of curative effect on tumour and the mechanisms of turnout formation. This paper also presents some prospects on the application of proteomics in heavv ion therapy.  相似文献   
8.
综述了DNA辐射损伤导致的细胞阻遏于G1期以及在该时期对DNA的修复活动, 提出了较大剂量辐射诱导的三磷酸腺苷不足导致细胞凋亡的假说, 并分析了细胞走向凋亡与修复的辨正关系。 DNA damage induced by irradiation,which makes the cell arrested at G1 stage and DNA repair being activated in this stage,are summarized. It is proposed that the deficiency of adenosine triphosphate which is induced by the larger irradiation dose, induces cell apoptosis. And the relationship of cell selecting repair and apoptosis is also analyzed.  相似文献   
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