This paper proposes a new type of molecular device that is able to act as an inverse proton sponge to slowly decrease the pH inside a reaction vessel. This makes the automatic monitoring of the concentration of pH‐sensitive systems possible. The device is a composite formed of an alkyl chloride, which kinetically produces acidity, and a buffer that thermodynamically modulates the variation in pH value. Profiles of pH versus time (pH–t plots) have been generated under various experimental conditions by computer simulation, and the device has been tested by carrying out automatic spectrophotometric titrations, without using an autoburette. To underline the wide variety of possible applications, this new system has been used to realize and monitor HCl uptake by a di‐copper(II) bistren complex in a single run, in a completely automatic experiment. 相似文献
Several natural biotite samples were studied by M.E., electron microprobe and XRD. The M.E. parameters allow to group them
as oxybiotites, normal biotites, chloritized biotites and muscovitized biotites. Mechanisms of oxybiotite formation and processes
undergone by the rocks are discussed. 相似文献
Lipophilic conjugates of the antitumor drug methotrexate (MTX) with lipoamino acids (LAAs) have been previously described as a tool to enhance MTX passive entrance into cells, overcoming a form of transport resistance which makes tumour cells insensitive to the antimetabolite. A knowledge of the mechanisms of interaction of such lipophilic derivatives with cell membranes could be useful for planning further lipophilic MTX derivatives with an optimal antitumour activity. To this aim, a calorimetric study was undertaken using a biomembrane model made from synthetic 1,2-dipalmitoyl-glycero-3-phosphocholine (DPPC) multilamellar liposomes. The effects of MTX and conjugates on the phase transition of liposomes were investigated using differential scanning calorimetry.
The interaction of pure MTX with the liposomes was limited to the outer part of the phospholipid bilayers, due to the polar nature of the drug. Conversely, its lipophilic conjugates showed a hydrophobic kind of interaction, perturbing the packing order of DPPC bilayers. In particular, a reduction of the enthalpy of transition from the gel to the liquid crystal phase of DPPC membranes was observed. Such an effect was related to the structure and mole fraction of the conjugates in the liposomes.
The antitumour activity of MTX conjugates was evaluated against cultures of a CCRF–CEM human leukemic T-cell line and a related MTX resistant sub-line. The in vitro cell growth inhibitory activity was higher for bis(tetradecyl) conjugates than for both the other shorter- and longer-chain derivatives. The biological effectiveness of the various MTX derivatives correlated very well with the thermotropic effects observed on the phase transition of DPPC biomembranes. 相似文献
We report studies on the structure of the metallic core and the alkyl cap layer in monolayer protected gold nanoparticles having sizes down to 1.6 nm. These particles are obtained by laser ablating gold targets in alkane-thiol solutions at different concentrations. The electronic structure of gold core and the vibrational properties of the capping hydrocarbon chains reveal effects connected with the nanosized nature of the particles. 相似文献
For 30 years the Lempel–Ziv factorization LZx of a string x = x[1..n] has been a fundamental data structure of string processing, especially valuable for string compression and for computing
all the repetitions (runs) in x. Traditionally the standard method for computing LZx was based on Θ(n)-time (or, depending on the measure used, O(n log n)-time) processing of the suffix tree STx of x. Recently Abouelhoda et al. proposed an efficient Lempel–Ziv factorization algorithm based on an “enhanced” suffix array – that is, a suffix array SAx together with supporting data structures, principally an “interval tree”. In this paper we introduce a collection of fast
space-efficient algorithms for LZ factorization, also based on suffix arrays, that in theory as well as in many practical
circumstances are superior to those previously proposed; one family out of this collection achieves true Θ(n)-time alphabet-independent processing in the worst case by avoiding tree structures altogether.
The work of the first and third authors was supported in part by grants from the Natural Sciences & Engineering Research Council
of Canada. 相似文献