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排序方式: 共有47条查询结果,搜索用时 31 毫秒
1.
František Kvasnička Rudolf Ševčík Michal Voldřich Jana Krátká 《Central European Journal of Chemistry》2004,2(3):417-424
A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary
supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5
minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose
in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed
method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running
cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC. 相似文献
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The reaction 125Te(d, p)126Te has been studied at a bombarding energy of 7.5 MeV in order to get information about the 126Te nuclear level scheme. Forty-two levels were observed below an excitation energy of 5.1 MeV. The angular distributions of 25 of the emitted proton groups were compared with DWBA calculations to determine the angular momentum of the captured neutrons. Contributions from more than one ln value were observed for two of the transitions. Transition strengths were extracted and compared to pairing-theory calculations. 相似文献
6.
Juliana S Luz Celso RR Ramos Márcia CT Santos Patricia P Coltri Fernando L Palhano Debora Foguel Nilson IT Zanchin Carla C Oliveira 《BMC biochemistry》2010,11(1):22
Background
The archaeal exosome is formed by a hexameric RNase PH ring and three RNA binding subunits and has been shown to bind and degrade RNA in vitro. Despite extensive studies on the eukaryotic exosome and on the proteins interacting with this complex, little information is yet available on the identification and function of archaeal exosome regulatory factors. 相似文献7.
Kvasnička F Copíková J Sevčík R Václavíková E Synytsya A Vaculová K Voldřich M 《Electrophoresis》2011,32(9):1090-1093
Phytic acid (PA) and lower inositolphosphates (InsP(n) ) is the main storage form of phosphorus in grains or seeds. The content of PA and InsP(n) in different varieties of barley was analyzed by capillary isotachophoresis and online-coupled capillary isotachophoresis with CZE. The electrolytes (in demineralized water) for the isotachophoretic analysis consisted of 10?mM HCl, 14?mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading) and 10?mM citric acid (terminating). The optimized electrolytes for the online coupling isotachophoresis with zone electrophoresis analysis were mixtures of 5?mM HCl, 7?mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (leading), 20?mM citric acid, 10?mM glycylglycine, and 0.1% 2-hydroxyethylcellulose (background) and 10?mM citric acid (terminating). PA and all studied InsP(n) were separated within 25?min and detected by a conductivity detector. Simple sample preparation (acidic extraction), sufficient sensitivity, speed of analysis, and low running cost are important attributes of the electrophoretic methods. The method was used for the determination of PA and InsP(n) in barley varieties within an ongoing research project. 相似文献
8.
Ivan Hung Julien Trbosc Gina L. Hoatson Robert L. Vold Jean-Paul Amoureux Zhehong Gan 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2009,201(1):81-86
The multiple-quantum magic-angle spinning (MQMAS) and satellite-transition magic-angle spinning (STMAS) experiments refocus second-order quadrupolar broadening of half-integer quadrupolar spins in the form of two-dimensional experiments. Isotropic shearing is usually applied along the indirect dimension of the 2D spectra such that an isotropic projection free of anisotropic quadrupolar broadening can be obtained. An alternative shear transformation by a factor equal to the coherence level (quantum number) selected during the evolution period is proposed. Such a transformation eliminates chemical shift along the indirect dimension leaving only the second-order quadrupolar-induced shift and anisotropic broadening, and is expected to be particularly useful for disordered systems. This transformation, dubbed Q-shearing, can help avoid aliasing problems due to large chemical shift ranges and spinning sidebands. It can also be used as an intermediate step to the isotropic representation for expanding the spectral window of rotor-synchronized experiments. 相似文献
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Counter-rotating open rotor acoustic measurements were processed using a two-shaft Vold–Kalman order tracking filter, providing new insight into the complicated noise generation mechanisms of this type of system. The multi-shaft formulation of the Vold–Kalman filter can determine a time-accurate output of shaft order tones associated with each rotor, even as the rotation rate of the two rotors varies. This is a major improvement over the usual short time Fourier transform method for many applications. It was found that the contribution from each rotor to the individual tones varies strongly as a function of shaft order and operating condition. The order tracking filter is also demonstrated as a robust tool for separating the tonal and broadband components of a signal for which the usual shaft phase averaging methods fail. 相似文献
10.
Renata Monteiro-Maia Maria B Ortigão-de-Sampaio Rosa T Pinho Luiz RR Castello-Branco 《Journal of immune based therapies and vaccines》2006,4(1):1-6
We hypothesize that the energy strategy of a cell is a key factor for determining how, or if, the immune system interacts
with that cell. Cells have a limited number of metabolic states, in part, depending on the type of fuels the cell consumes.
Cellular fuels include glucose (carbohydrates), lipids (fats), and proteins. We propose that the cell's ability to switch
to, and efficiently use, fat for fuel confers immune privilege. Additionally, because uncoupling proteins are involved in
the fat burning process and reportedly in protection from free radicals, we hypothesize that uncoupling proteins play an important
role in immune privilege. Thus, changes in metabolism (caused by oxidative stresses, fuel availability, age, hormones, radiation,
or drugs) will dictate and initiate changes in immune recognition and in the nature of the immune response. This has profound
implications for controlling the symptoms of autoimmune diseases, for preventing graft rejection, and for targeting tumor
cells for destruction. 相似文献