Two new ent-kaurane diteroenoids from Isodon excisoides

Two new ent-kaurane diterpenoids taihangexcisoidesin A and B(1 and 2),were isolated from the EtOAc extract of the leaves of Isodon excisoides.Their structures were determined on the basis of spectroscopic methods.     

Screening and docking studies of natural phenolic inhibitors of carbonic anhydrase Ⅱ

Carbonic anhydrase Ⅱ (CAⅡ) is an important enzyme complex with Zn2+,which is involved in many physiological and pathological processes, such as calcification, glaucoma and tumorigenicity. In order to search for novel inhibitors of CAⅡ, inhibition assay of carbonic anhydrase Ⅱ was performed, by which seven natural phenolic compounds, including four phenolics (grifolin, 4-O-methyl-grifolic acid, grifolic acid, and isovanillic acid) and three flavones (eriodictyol, quercetin and puerin A), showed in-hibitory a...     

5种重要农艺性状基因在水稻重组自交系群体中的定位

通过抗病虫害水稻品系“B5”与籼稻品种“明恢63”为亲本进行杂交，得到一个187个F8代的重组自交系群体。利用重组自交系群体构建的分子连锁图，对水稻抽穗期、株高、穗长、剑叶长和剑叶宽的性状进行了QTL分析，分别检测到影响抽穗期、株高、穗长、剑叶长和剑叶宽的农艺性状2、2、4、4、4个QTLs，并分别解释各表型性状总变异的50．6％、74．9％、34．1％、47．3％和46．2‰．其中，控制抽穗期的Hd-6基因，控制株高的Ph7基因和控制剑叶长的Fll-2b基因分别是效应值较大的主效座位，其余的为微效基因座位。相关性状的QTLs大多聚集在染色体的相近或相同区间，表现出成簇分布的现象。这些重要农艺性状的分析，可以为水稻分子标记辅助选择育种提供有用的遗传信息。     

Three New Diterpenoids from Euphorbia wallichii

Three new abietane diterpene lactones, 3α-hydroxyjolkinolide A (1), ent-8α, 14β-dihydroxy-13(15)-ene-16(12α)-abietanolide (2) and ent-8α,14α-dihydroxy-13(15)-ene-16(12α)-abietanolide (3) as well as a known abietane diterpene jolkinolide A (4) were isolated from the roots of Euphorbia wallichii. Their structures were elucidated on the basis of spectroscopic analysis.     

A New Triterpenoid Saponin and a New Glycoside from Epigynum aurilum

A new triterpenoid saponin with novel sugar moiety (1), a new trisaccharide derivative (2), as well as a known monosaccharide derivative (3), were isolated from the dried stem of Epigyhum aurilum. The structures of compounds 1 and 2 were determined by MS and NMR spectram analyses.     

Radiometric and fluorimetric determination of aminosilanes and protein covalently bound to thermally pretreated glass substrates

Derivatization of solid soda-lime glass spheres with aminosilanes and the stability of these groups near physiological pH in flow streams of aqueous buffered solutions are described. The presence of immobilized and adsorbed amines in the nanomolar range is confirmed by using two independent methods, one based on a radiotracer and the other on the fluorescent marker, fluorescamine. A method for covalently attaching bovine serum albumin to the beads is described.     

Modeling the concentration dependence of the methanol self-diffusivity in faujasite systems: comparison with the liquid phase

Molecular dynamics simulations were performed to understand further the concentration dependence of the self-diffusion of methanol in the faujasite zeolite systems. The evolution of the self-diffusivity was investigated as a function of coverage for DAY and NaY systems to study the effect of both the pore confinement and the presence of the extraframework cations within the supercage. It was found that the self-diffusivity decreases with loading for DAY, whereas for NaY it passes through a maximum at intermediate coverage, in agreement with pulse-field gradient NMR and quasi elastic neutron scattering data reported in similar systems. The activation energies of the methanol diffusion corresponding to a combination of both intra- and intercage motions were evaluated as a function of the coverage. The simulated trends are interpreted on the basis of the predominant interactions which take place in both systems. Finally, the preferential arrangement of the adsorbate molecules are provided and compared with those simulated in the liquid phase. For the fully loaded materials, it was seen that the methanol molecules form a one-dimensional hydrogen-bonded chain along the channels in DAY whereas only dimers are present in NaY.     

Molecular dynamics simulation of the cation motion upon adsorption of CO2 in Faujasite zeolite systems

Molecular Dynamics simulations have been carried out in NaX and NaY Faujasite systems to deepen understanding of the cation rearrangement during the CO2 adsorption process suggested by our recent diffusivity measurements. This study is a major contribution since the rearrangement of the cations in Faujasite, the most promising adsorbent for CO2 storage, can represent a significant breakthrough in understanding the adsorption and diffusion processes at the mircroscopic scale. For NaY, it has been shown that at low and intermediate loadings, SII cations can migrate toward the center of the supercage due to strong interactions with the adsorbates, followed by a hopping of SI'cation from the sodalite cage into the supercage to fill the vacant SII site. The SI cations are only displaced at a higher loading, leading to cation de-trapping out of the double six rings into the vacant SI' sites. For NaX, the SIII' cations which occupy the most accessible adsorption sites move significantly upon coordination to the carbon dioxide molecules. The SI' and SII cations remain consistently located in their initial sites whatever the loading. Indeed, the most probable migration mechanism involves SIII' cation displacements into nearby vacant SIII' sites.