Multi-objective evacuation routing in transportation networks

In this paper, the optimal design and analysis of evacuation routes in transportation networks is examined. An methodology for optimal egress route assignment is suggested. An integer programming (IP) formulation for optimal route assignment is presented, which utilizes M/G/c/c state dependent queueing models to cope with congestion and time delays on road links. M/G/c/c simulation software is used to evaluate performance measures of the evacuation plan: clearance time, total travelled distance and blocking probabilities. Extensive experimental results are included.     

Constrained kernelized partial least squares

Nonlinear kernel methods have been widely used to deal with nonlinear problems in latent variable methods. However, in the presence of structured noise, these methods have reduced efficacy. We have previously introduced constrained latent variable methods that make use of any available additional knowledge about the structured noise. These methods improve performance by introducing additional constraints into the algorithm. In this paper, we build upon our previous work and introduce hard‐constrained and soft‐constrained nonlinear partial least squares methods using nonlinear kernels. The addition of nonlinear kernels reduces the effects of structured noise in nonlinear spaces and improves the regression performance between the input and response variables. Copyright © 2014 John Wiley & Sons, Ltd.     

The contribution of 2N photon absorption in C(γ,2N) reactions for Eγ = 150–400 MeV

The ¹²C(γ,pn) and ¹²C(γ,pp) reactions have been studied using tagged photons of energy E_γ = 150–400 MeV. Recoil momentum distributions are compared to the results of Monte Carlo calculations based on a two-nucleon photon absorption model and two different phase space models. The ¹²C(γ,pn) data at low missing energy are consistent with absorption on 1p² and 1s1p nucleon pairs.     

Tandem purification of mouse IgM monoclonal antibodies produced in vitro using anion-exchange and gel fast protein liquid chromatography

A tandem chromatographic procedure was used to isolate rapidly mouse IgM monoclonal antibodies produced by cultivation of hybridomas in vitro. Hybridoma culture supernatants containing mouse IgM monoclonal antibodies were first chromatographed on an anion-exchange Mono Q column connected to a fast protein liquid chromatography system. This anion-exchange step offers the advantage of obtaining IgM antibodies in a concentrated form. The IgM-rich fractions from the Mono Q column were then injected on a gel filtration Superose 6 column equilibrated with a low-ionic strength buffer and eluted with a high-ionic strength buffer. Assessment of the purity of isolated IgM monoclonal antibodies was performed by sodium dodecyl sulphate polyacrylamide gel electrophoresis together with a Coomassie Brillant Blue R 250 staining technique. Assessment of the immunoreactivity of isolated IgM monoclonal antibodies was performed by an enzyme linked immunosorbent assay using a solid phase adsorbed antigen against which IgM monoclonal antibodies were directed. The chromatographic procedures described allows the rapid isolation of mouse IgM monoclonal antibodies produced in vitro at a high degree of purity and in an immunoreactive state.     

Anti-malarial, anti-algal, anti-tubercular, anti-bacterial, anti-photosynthetic, and anti-fouling activity of diterpene and diterpene isonitriles from the tropical marine sponge Cymbastela hooperi

In an investigation into their potential ecological role(s), a group of mainly diterpene isonitriles, nine in total, isolated from the tropical marine sponge Cymbastela hooperi, and the sesquiterpene axisonitrile-3, isolated from the tropical marine sponge Acanthella kletra, were evaluated in a series of bioassays including anti-fouling, anti-algal, anti-photosynthetic, anti-bacterial (Gram +ve and -ve), anti-fungal, and anti-tubercular. The results of these assays showed that all of the tested compounds, with the exception of diterpene 9, were active in at least two of the applied test systems, with axisonitrile-3 (10) and diterpene isonitrile 1 being the two most active compounds overall, closely followed by diterpene isonitrile 3. Based on the results of the photosynthetic study a molecular modelling investigation was undertaken with all of the compounds used in that study. The results showed a positive correlation between reduction in photosynthetic activity and the interaction of the modelled compounds with a potential enzyme active site.     

An O (n2) heuristic algorithm for the directed Steiner minimal tree problem

This paper describes the design of an O(N²) heuristic algorithm for the Directed Steiner Minimal Tree (DSMT) problem in the plane. The algorithm employs the Delaunay triangulation, a minimum spanning arborescence algorithm, and a triangulation of the arborescence to develop solutions to the DSMT problem. Example applications are also included.     

Measurement of the electric form factor of the neutron at Q2 = 0.3-0.8 (GeV/ c) 2 ⋆

The electric form factor of the neutron, G_E,n, has been measured at the Mainz Microtron by recoil polarimetry in the quasielastic D(¯e, e¯n)p reaction. Three data points have been extracted at squared four-momentum transfers Q ² = 0.3, 0.6 and 0.8 (GeV/c)². Corrections for nuclear binding effects have been applied.This revised version was published online in March 2005. In the previous version, the email address of one author was inadvertently assigned to multiple authors.     

Structure-enantioselectivity effects in 3,4-dihydropyrimido[2,1-b]benzothiazole-based isothioureas as enantioselective acylation catalysts

The catalytic activity and enantioselectivity in the kinetic resolution of (±)-1-naphthylethanol with a range of structurally related 3,4-dihydropyrimido[2,1-b]benzothiazole-based catalysts is examined. Of the isothiourea catalysts screened, (2S,3R)-2-phenyl-3-isopropyl substitution proved optimal, giving good levels of selectivity in the kinetic resolution of a number of secondary alcohols (S values up to >100 at ~50% conversion). Low catalyst loadings (0.10-0.25 mol%) of the optimal isothiourea can be used to generate enantiopure alcohols (>99% ee) in good yields.