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排序方式: 共有924条查询结果,搜索用时 16 毫秒
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Eveline Vriens Dr. Dries De Ruysscher Dr. Angus N. M. Weir Dr. Sofie Dekimpe Dr. Gert Steurs Ahmed Shemy Leentje Persoons Dr. Ana Rita Santos Dr. Christopher Williams Prof. Dirk Daelemans Prof. Matthew P. Crump Prof. Arnout Voet Prof. Wim De Borggraeve Prof. Eveline Lescrinier Prof. Joleen Masschelein 《Angewandte Chemie (International ed. in English)》2023,62(34):e202304476
Bacterial trans-acyltransferase polyketide synthases (trans-AT PKSs) are modular megaenzymes that employ unusual catalytic domains to assemble diverse bioactive natural products. One such PKS is responsible for the biosynthesis of the oximidine anticancer agents, oxime-substituted benzolactone enamides that inhibit vacuolar H+-ATPases. Here, we describe the identification of the oximidine gene cluster in Pseudomonas baetica and the characterization of four novel oximidine variants, including a structurally simpler intermediate that retains potent anticancer activity. Using a combination of in vivo, in vitro and computational approaches, we experimentally elucidate the oximidine biosynthetic pathway and reveal an unprecedented mechanism for O-methyloxime formation. We show that this process involves a specialized monooxygenase and methyltransferase domain and provide insight into their activity, mechanism and specificity. Our findings expand the catalytic capabilities of trans-AT PKSs and identify potential strategies for the production of novel oximidine analogues. 相似文献
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Dr. Tobias W. Giessen Florian Altegoer Annika J. Nebel Roman M. Steinbach Dr. Gert Bange Prof. Dr. Mohamed A. Marahiel 《Angewandte Chemie (International ed. in English)》2015,54(8):2492-2496
The incorporation of non‐proteinogenic amino acids represents a major challenge for the creation of functionalized proteins. The ribosomal pathway is limited to the 20–22 proteinogenic amino acids while nonribosomal peptide synthetases (NRPSs) are able to select from hundreds of different monomers. Introduced herein is a fusion‐protein‐based design for synthetic tRNA‐aminoacylation catalysts based on combining NRPS adenylation domains and a small eukaryotic tRNA‐binding domain (Arc1p‐C). Using rational design, guided by structural insights and molecular modeling, the adenylation domain PheA was fused with Arc1p‐C using flexible linkers and achieved tRNA‐aminoacylation with both proteinogenic and non‐proteinogenic amino acids. The resulting aminoacyl‐tRNAs were functionally validated and the catalysts showed broad substrate specificity towards the acceptor tRNA. Our strategy shows how functional tRNA‐aminoacylation catalysts can be created for bridging the ribosomal and nonribosomal worlds. This opens up new avenues for the aminoacylation of tRNAs with functional non‐proteinogenic amino acids. 相似文献
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Stoyan I. Karakashev Klaus W. Stöckelhuber Roumen Tsekov Chi M. Phan Gert Heinrich 《Advances in colloid and interface science》2014
This work shows a successful example of coupling of theory and experiment to study the tribology of bubble rubbing on solid surface. Such kind of investigation is reported for the first time in the literature. A theory about wetting film intercalated between bubble and moving solid surface was developed, thus deriving the non-linear evolution differential equation which accounted for the friction slip coefficient at the solid surface. The stationary 3D film thickness profile, which appears to be a solution of the differential equation, for each particular speed of motion of the solid surface was derived by means of special procedure and unique interferometric experimental setup. This allowed us to determine the 3D map of the lift pressure within the wetting film, the friction force per unit area and the friction coefficient of rubbing at different speeds of motion of the solid surface. Thus, we observed interesting tribological details about the rubbing of the bubble on the solid surface like for example: 相似文献
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Dr. Eddy W. T. Yemeli Dr. Graeme R. Blake Dr. Alexios P. Douvalis Prof. Thomas Bakas Gert O. R. Alberda van Ekenstein Dr. Petra J. van Koningsbruggen 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(72):16766-16776
The iron(III) spin-crossover compounds [Fe(Hthsa)(thsa)] ⋅ H2O ( 1 ), [Fe(Hth5Clsa)(th5Clsa)2] ⋅ H2O ( 2 ), and [Fe(Hth5Brsa)(th5Brsa)2] ⋅ H2O ( 3 ) (H2thsa=salicylaldehyde thiosemicarbazone, H2th5Clsa=5-chlorosalicylaldehyde thiosemicarbazone, and H2th5Brsa=5-bromosalicylaldehyde thiosemicarbazone) have been synthesized and their spin-transition properties investigated by magnetic susceptibility, Mössbauer spectroscopy, and differential scanning calorimetry measurements. The three compounds exhibit an abrupt spin transition with a thermal hysteresis effect. The more polarizable the substituent on the salicylaldehyde moiety, the more complete is the transition at room temperature with an increased degree of cooperativity. The molecular structures of 1 and 2 in the high-spin state are revealed. The occurrence of the light-induced excited-spin-state trapping phenomenon appears to be dependent on the substituent incorporated into the 5-position of the salicylaldehyde subunit. Whereas the compounds with an electron-withdrawing group (-Br or -Cl) exhibit light-induced trapped excited high-spin states with great longevity of metastability, the halogen-free compound does not, even though strong intermolecular interactions (such as hydrogen-bonding networks and π stacking) operate in the system. For compound 2 , the surface level of photoconversion is less than 35 %. In contrast, compound 3 displays full photoexcitation. 相似文献
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