壳聚糖铜(Ⅱ)配位与氧化控制降解寡糖的GFC色谱研究

在壳聚糖溶液中加入铜离子使形成壳聚糖铜配合物,通过IR,UV,元素分析及热重分析等对壳聚糖铜配合物进行表征。用H₂O₂对形成的壳聚糖Cu(Ⅱ)配合物进行氧化降解,测定了降解产物寡糖的平均数均分子量和分子量分布。结果表明壳聚糖铜配合物在升高温度时,在过氧化氢的存在下降解迅速,降解从大分子量范围开始,降解寡糖分子量分布在温度和降解时间相同条件下大大窄于目前常用的盐酸和纯过氧化氢氧化降解方法。分子量分布指数与降解寡糖的平均数均分子量有关,当高于10个寡聚糖数(DP)时,DP越小,分子量分布指数越小。     

壳聚糖锰(Ⅱ)配位与氧化控制降解寡糖的分子量分布

Complexes of chitosan with Mn(Ⅱ) were prepared by adding Mn(OAc)₂·4H₂O to Chitosan solution. IR, elemental analysis and TG analysis were used to character the complex. The results showed that there were coordinate bands formed. H₂O₂ was used to degrade chitosan-Mn(Ⅱ) complex, and the molecular distribution of degraded products were investigated after eliminating Mn(Ⅱ) ions using the cation exchange resin column. The result suggested that the Chitosan could be degraded rapidly, the degradation started from higher molecular weight range, the molecular weight distribution of oligosaccharides was much more narrower than that of degradated products from common methods such as hydrolysis, acidic and oxidizing methods. The index of molecular weight distribution was changed with the average degradability. When exceeding 10 oligosaccharides, the smaller of the DP, the smaller of the index.