Investigation of Antibacterial Activity of Two Kinds of Novel Schiff Bases on Escherichia coli by Microcalorimetry

The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff basecompounds(H_2L~(3')and H_2L~3)on Escherichia coli,trying to obtain the action on both of multiplying bacteria andnon-multiplying bacteria at one experiment.The metabolic power-time curves of the bacteria treated with the com-pounds were obtained,and the thermokinetic parameters were analyzed,from which the antibacterial activities ofthese compounds were evaluated.The results showed that both of the two compounds have good activity on aerobicmultiplying metabolism of E.coli,with the value of IC_(50)75.8 and 168.8 mg/L respectively,but have not effectiveaction on fermentation metabolism of E.coli.The action of the compounds on the non-multiplying metabolism wasinvestigated by taking the heat output of E.coli in the stationary phase as the guideline of the activity.The value ofMSC_(50)(minimum stationary-cidal concentration 50)of them is 118 and 187.5 mg/L,respectively.So,H_2L~(3')hasstronger antibacterial action on E.coli than H_2L~3 either for multiplying bacteria or non-multiplying bacteria,andtheir activity on the aerobic multiplying bacteria of E.coli is mainly shown.It does strongly suggest that the calo-rimetric method should play an important role in the fight against the drug-resistant bacteria.     

底部加热空气层自然对流的可视化实验研究

本文对底部加热的水平空气层的自然对流换热进行了可视化实验研究,采用实时全息干涉照相技术获得了温度场的激光干涉图及流场的烟可视化图象。实验结果表明,这种水平空气层自然对流换热是非稳态的,并且在空间上是三维的。     

基质与样品的相互作用对于傅立叶变换离子回旋共振质谱测定低分子量多肽的影响

通过比较两种极性差异较大的基质2,5-二羟基苯甲酸(DHB)和2’,6’-二羟基苯乙酮(DHAP)按不同比例混合时,AngiotensinⅡ的基质辅助激光解析电离-傅立叶变换离子回旋共振质谱(MALDI-FT/ICRMS)谱图的不同,并结合基质-AngiotensinⅡ在不同结晶方式下的共结晶和基质晶体的扫描电镜照片,发现基质为10μmol/L DHB和15μmol/L DHAP以体积比4:1组成的混合物时,基质结晶为致密的层状结构,而以薄层法与AngiotensinⅡ生成的共结晶,AngiotensinⅡ在基质晶体上面形成分散的柱状小晶体,此时得到的MALDI-FT/ICRMS质谱图优于干滴法。     

盾构作业区域湿热环境的研究

通过对掘进中的盾构及隧道内的温度和湿度的实地测量,本文针对盾构施工过程中在其作业区域存在的高温高湿等恶劣的工作环境问题,就其形成的原因和热湿传递的特性进行了分析,还对其中的流场进行了初步的数值模拟,提出了改善盾构作业区域内工作环境的措施。     

场协同与对流换热的稳定性

本文提出了流动与换热中的各种场的协同,会影响流动和换热的稳定性这一新观点。通过对带有倾角的方形空间自然对流换热及具有对称射流的方形空间内混合对流换热问题的数值计算可以发现,这两个流动换热的例子中,流场、温度场、重力场的协同能够影响其流动与换热的稳定性,这初步验证了本文提出的这一观点。     

稀土元素分离检测技术新进展

对2003年以来稀土元素分离检测技术的新进展进行了评述，重点集中于各种新型分离技术、新型检测技术及其在稀土元素分析中的应用。对于离子液体、纳米材料、离子印迹聚合物等新型材料以及浊点萃取、膜萃取、毛细管电泳等新型技术在稀士元素分离中的应用进行了详细讨论，并对中子活化分析、等离子体原子发射光谱和等离子体质谱在稀土元素检测中的应用进行了综述，重点讨论了等离子体质谱技术的应用。在回顾稀土元素分离检测技术进展的同时，对其在未来几年的发展趋势进行了评述。     

微波辐射下一锅法合成2-氨基-6-溴-4-芳基-5H-茚并[1,2-b]吡啶-3-腈衍生物

以芳香醛、4-溴茚酮、丙二腈和醋酸铵为原料,分别用微波辐射和传统加热法,一步法合成7种未见文献报道的2-氨基-6-溴基-4-芳基-5H-茚并[1,2-b]吡啶-3-腈衍生物.对比两种方法,在常压下微波具有反应时间短、产率较高等特点.所有化合物均经IR,1HNMR和元素分析确定.     

溶剂对4-叠氮苯甲酸共配叠氮铜配合物磁学性质影响的理论研究

基于DFT-BS方法,在不同泛函方法和基组下研究μ-1,1叠氮、4-叠氮苯甲酸、溶剂共配的叠氮铜配合物[Cu2(4-aba)3(N3)3(CH3OH)3]2-的磁学性质。结果显示,在B2PLYP/TZVP水平下计算得到顺磁中心间的磁耦合常数为33.48 cm-1,与实验值33.80 cm-1最吻合,可准确描述其磁学性质。配合物[Cu2(4-aba)3(N3)3(CH3OH)3]2-的分子磁轨道主要贡献来自于顺磁中心Cu(1)、Cu(2)的3dx2-y2轨道、N3-离子的π型轨道、4-叠氮苯甲酸的π型轨道和溶剂甲醇氧原子的p轨道。在4-叠氮苯甲酸共配的叠氮铜配合物中引入共配溶剂水及醇类,随溶剂R基供电子效应的增加,反铁磁性部分对磁耦合常数的贡献减小,其磁耦合常数J值随之增大。     

高分子化学双语教学的改革初探与感悟

高分子化学作为一门贴近大众生活的化学类专业课程,是在四大化学(有机化学、无机化学、物理化学、分析化学)基础上,进一步培养高分子专业学生在掌握分子结构设计、合成路线设计、结构性能研究等方面理论知识,能够进一步解决实际问题的能力的课程。高分子化学在基础化学以及高分子物理、聚合物加工与成型等专业课程的设立中起到了很好的桥梁作用。同时,为了适应面向现代化,面向世界,面向未来的教育理念,双语教学模式的建立是高等教育改革中的一个热点问题之一,也是高等教育全球化的必然趋势。因此,作者基于高分子化学双语课程课堂教学经验,对如何提升学生的学习兴趣,提高双语教学质量,增强学生全球竞争力等方面做了改革初探。     

Affinity switching for lysozyme and dual-responsive microgels by stopped-flow technique: Kinetic control and activity evaluation

The use of proteins as therapeutics in nanomedicine is an emerging research field and has developed rapidly.However,proteins are always vulnerable to renal excretion or digestion by the proteolytic system in vivo,which limits their usage to a large extent.Although biocompatible polymers have been covalently linked to proteins to protect them from recognition by the immune system and prolong their circulation time,the biological activity of them is sometimes decreased.To fill this gap,physical isolation,wrapping,or encapsulation techniques are employed.Up to now,various mature examples were reported,but the whole time scales for guest molecules loading and releasing,especially the initial rapid loading process,were rarely mentioned.Herein,a series of dual-responsive poly(N-isopropylacrylamide-co-methacrylic acid)(P(NIPAM-co-MAA)) microgels were synthesized and employed to investigate the kinetics of in situ complexation and release of lysozyme under external stimuli modulation upon a stopped-flow apparatus,which was suitable for rapid dynamic monitoring.Close inspection of the adsorption kinetics during the early stages( 50 s) revealed that the initial microgel collapse occurred within ~1 s,with more rapid transitions being observed when higher lysozyme concentrations were targeted.All the dynamic traces could be well fitted with a double exponential function,suggesting a fast(τ1) and a slow(τ2) relaxation time,respectively.Then,the kinetics of releasing bound lysozyme from microgels was carried on by utilizing the p H-responsive property,and the evaluation of the activity of released lysozyme was synchronously measured in a Micrococcus lysodeikticus(M.lysodeikticus) cell suspension.The corresponding relaxation time(τ) was also calculated by fitting the recorded dynamic traces.We speculate that this work can provide basic dynamics data and theoretical basis for microgels based nanocarriers to be used for protein delivery,controlled release,and possible chemical separation.