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1.
辐照诱导的人正常肝细胞系HL-7702细胞延迟效应   总被引:1,自引:0,他引:1  
利用X射线辐照人正常肝细胞系HL-7702细胞, 运用胞质分离阻滞微核法实验检测细胞微核率,AnnexinV FITC细胞凋亡检测试剂盒检测细胞凋亡率, 细胞微核率和凋亡率随着辐照剂量的增加而显著增加。X射线照射后细胞传代培养, 第7代时不同剂量辐照后子代细胞微核率和凋亡率同未辐照细胞相比已无明显区别。 对不同剂量辐照后传代7代的细胞再次照射2.5 Gy的相同剂量,发现它们细胞微核率和凋亡率存在明显差异,即初次受辐照剂量高的细胞, 再次以相同剂量辐照后的微核率和凋亡率也高. 这些结果表明,X射线辐照导致了HL-7702细胞基因组不稳定性这一辐射延迟效应,再次辐照使得辐射的延迟效应得以明显的表现。 Human normal liver cell line HL-7702 cells were irradiated with different doses of X rays. Micronucleus and apoptosis rates in the irradiated cells were measured with cytokinesis block micronucleus method and Annexin V FITC apoptosis detection kit, respectively. Experimental data showed that the micronucleus and apoptosis rates increased obviously with increasing irradiation dose. After seven population doublings, the micronucleus and apoptosis rates of the cells surviving exposure to the X rays reduced to the same levels as non irradiated control cells; the progenies of the cells were secondly exposed to X rays at the same dose of 2.5 Gy. We found that the progenies of the cells surviving the first irradiations of the various doses showed markedly differential micronucleus frequencies and apoptotic rates. Although the same dose of 2.5 Gy was applied in the second irradiations, the micronucleus frequencies and apoptotic rates of the progenies of the cells initially exposed at higher doses were significantly higher than the others. These results indicate that X rays lead to genomic instability in HL 7702 cells, which is an important manifestation of radiation induced delayed effect, and a second radiation stimulus makes the delayed effect in the progeny of the previously irradiated cells be expressed obviously.  相似文献   
2.
分别制备经1 Gy C离子辐照和未经辐照人肝癌HepG2细胞的总蛋白样品, 采用固相pH梯度双向凝胶电泳技术进行蛋白分离, 用ImageMaster 2D双向电泳凝胶图像分析软件分析数字化图谱。 结果显示, 差异表达的蛋白质点为17个, 其中1个仅在未经C离子辐照的HepG2细胞中表达, 8个蛋白质点在辐照后的细胞中表达上调, 8个蛋白质点在辐照后的细胞中表达下调。 建立了重复性较好和分辨率较高的分离HepG2细胞总蛋白的双向电泳技术。 实验结果表明, C离子辐照HepG2细胞后其蛋白质组发生了改变。  相似文献   
3.
多方过程几种定义说法等效性的证明   总被引:1,自引:0,他引:1  
多方过程,又名多变过程,在热力工程中有重要的实用价值.在普通物理热学教材中对于多方过程的讲解,一般是不加推导直接利用理想气体的绝热过程得出多方过程方程,有关多方过程的定义说  相似文献   
4.
信号因子通常能介导旁效应的发生。 利用高LET C离子辐照体外培养的人肝癌QGY-7703细胞, 检测辐照后不同时刻培养基中信号因子TGF-β1和NO的浓度, 并通过转移培养基法检测照射后不同时刻转移培养基对人肝L02旁细胞存活率和代谢活力的影响, 发现受照射细胞在时间与空间上调控着周围信号因子的浓度, 并且通过信号因子浓度的变化影响旁细胞的各种效应发生。 实验为旁效应的解释提供了新的实验数据。 Signaling factors usually play an important role in bystander effect. In this work, human hepatoma QGY 7703 cells in vitro were irradiated with high LET carbon ions. Concentrations of signaling factors such as TGF-β1 and NO were measured in the media of the irradiated QGY-7703 cells at different time points after irradiation. The conditioned media harvested at various times post irradiation were transferred to human hepatocyte L02 cells as bystander cells and then the influence of the conditioned media on survival fraction and cell viability of the bystander cells were determined. The results show that the irradiated cells regulate the concentration of the signaling factors released nearby themselves temporally and spatially, and the bystander cells response to the signaling factors differentially according to the concentration change. This work provides new basic data for exploring the bystander effect, especially caused by high LET radiation.  相似文献   
5.
Up to now, clinical trials of heavy-ion radiotherapy for superficially placed tumors have been carried out for six times and over 60 selected patients have been treated with 80--100 MeV/u carbon ions supplied by the Heavy Ion Research Facility in Lanzhou (HIRFL) at the Institute of Modern Physics, Chinese Academy of Sciences since November, 2006. A passive irradiation system and a dose optimization method for radiotherapy with carbon-ion beams have been developed. Experimental verification of longitudinally therapeutic dose distributions was conducted under the condition of simulating patient treatment in the therapy terminal at HIRFL. The measured depth-dose distributions basically coincide with the expected ones. These results indicate that the irradiation system and the dose optimization method are effective in the ongoing carbon-ion radiotherapy for shallow-seated tumors at HIRFL.  相似文献   
6.
采用实验与理论模拟相结合的方法,研究了氮化镓基绿光发光二极管(LED)中V坑对空穴电流分布的影响。首先,实验获得了V坑面积占比不同的3种样品;然后,建立数值模型,使得理论计算的外量子效率(EQE)及电压与实验测试的变化趋势相匹配,从而确立了所用数值模型的可信性。计算结果显示:V坑改变了空穴电流的分布,空穴电流密度在V坑处显著增加,在平台处明显减小。进一步的分析表明:V坑面积占比在0~10%范围内,V坑空穴电流占比与V坑面积占比之间呈近线性增长(斜率为2.06),但V坑空穴注入在整个空穴注入的过程中仍未占主导。  相似文献   
7.
先前的研究表明, 肿瘤细胞中survivin的高表达与细胞对高传能线密度(LET)射线的辐射抗性相关。 研究了survivin表达在高LET射线诱导的细胞凋亡中的作用, 发现抑制survivin表达对高LET C离子辐射诱导的Bcl-2和Bax表达没有明显的影响。 在高LET射线辐照中, survivin可能通过抑制caspase-3和-9活性的途径, 抑制了细胞凋亡。It has been proven that over expression of survivin in cancerous cell lines is related to the radioresistance of cells to high LET radiation in previous work. In this study, action mechanisms of survivin gene in apoptosis induced by high LET radiation were investigated. We found that inhibiting survivin by siRNA had no notable influence on Bcl-2 and Bax expressions induced by carbon ions. Survivin depressed cell apoptosis through the inhibition of the activities of caspase-3 and -9 possibly in cell apoptosis induced by high LET radiation.  相似文献   
8.
初步的IMP重离子治疗计划系统   总被引:1,自引:0,他引:1  
中国科学院近代物理研究所基于兰州重离子研究装置(HIRFL/HIRFL-CSR),在被动型束流配送系统下采用二维分层适形照射治疗技术开展重离子治癌临床试验研究。为了更好地利用重离子束在肿瘤放射治疗中的生物物理优势并保障重离子临床治疗试验的顺利实施,一个初级版本的重离子治疗计划系统已经设计完成。此计划系统是针对被动型束流配送系统下的二维分层适形照射治疗方式来进行设计的。介绍了此系统的设计框架、可提供的功能以及利用宽束算法进行剂量计算在此系统中的实现。通过人体仿真体模实验证实由该治疗计划系统给出的靶区计划剂量与实测剂量的偏差在5%之内。最后讨论了设计较为完备的重离子治疗计划系统仍需解决的问题。  相似文献   
9.
Effect of hydrogen(H_2) treatment during the GaN barrier growth on the electroluminescence performance of green In GaN/GaN single-quantum-well light-emitting diodes(LEDs) grown on Si substrates is experimentally investigated. We prepare two LED samples with different carrier gas compositions during the growth of GaN barrier. In the H_2 free LED, the GaN barrier is grown in full nitrogen(N_2) atmosphere. For the other H_2 treated LED, a mixture of N_2 and H_2 was used as the carrier gas. It is observed that V-shaped pits decrease in size after H_2 treatment by means of the scanning electron microscope. Due to the fact that the p–n junction interface would be closer to the p-GaN as a result of smaller V-shaped pits, the tunneling barrier for holes to inject into the In GaN quantum well would become thicker after H_2 treatment. Hence, the external quantum efficiency of the H_2 treated LED is lower compared to the H_2 free LED. However, LEDs would exhibit a better leakage behavior after H_2 treatment during the GaN barrier growth because of more effective blocking of the threading dislocations as a result of the H_2 etching at V-shaped pits.  相似文献   
10.
惠斯通电桥广泛应用于现代测量技术以及自动控制领域,本文从理论上分析了惠斯通电桥电压灵敏度与组成电路中各元件参数的关系,讨论了影响电桥电压灵敏度的几个关键因素,得出获得最大电压灵敏度的参数条件。分析结果表明:惠斯通电桥的电压灵敏度主要由桥路相邻两侧电阻的比值决定,等臂电桥具有最大灵敏度。同时,考虑到桥路输出电压与组成电路中的电阻相对变化率具有非线性,使用电阻应变片并采用半桥差动方式来搭建实验测试装置,以消除桥路电压非线性带来的影响。最后,利用自主搭建的半桥差动电桥对不同弯曲应变状态下的测量电压灵敏度进行了实验测试。实验结果表明,通过半桥差动连接方式,弯曲应变状态下桥路电压随应变程度呈线性关系  相似文献   
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