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以胆固醇琥珀酸单酯对壳聚糖进行仿生功能化改性, 并利用红外光谱和核磁共振波谱对改性后产物进行了表征. 通过接触角测试、 仿生矿化及细胞培养等方法研究了改性后产物的相关性能. 结果表明, 改性后的壳聚糖衍生物接触角有所增大. 在仿生矿化过程中, 随着时间的延长, 壳聚糖衍生物具有对沉积的钙磷盐的稳固作用. 在细胞培养过程中, 改性后的壳聚糖膜上细胞增殖明显. 噻唑蓝(MTT)比色法、 扫描电子显微镜(SEM)与激光共聚焦显微镜(CLSM)表征结果表明, 改性后的壳聚糖膜具有促进3T3细胞黏附、 伸展与增殖的效果. 相似文献
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从仿生角度出发, 制备了一系列液晶态胶原薄膜. 采用偏光显微镜、扫描电镜和原子力显微镜分别研究了各种膜的液晶织构和拓扑结构, 并通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法及细胞骨架荧光染色观察研究了各种膜对鼠成纤维细胞3T3的黏附、增殖以及形貌的影响. 结果表明, 胶原液晶薄膜呈现典型的胆甾型液晶织构, 与非液晶薄膜相比, 它不仅能更好地促进细胞的黏附、生长及增殖, 而且其拓扑结构对细胞生长具有接触引导效应. 相似文献
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Binary mixtures of 1,3-dialkylimidazolium based ionic liquids (ILs) and water were selected as solvent systems to investigate the solute-solvent and solvent-solvent interactions on the preferential solvation of solvatochromic indicators at 25℃. Empirical solvatochromic parameters, dipolarity/polarizability (π*), hydrogen-bond donor acidity (α), hydrogen-bond acceptor basicity (β), and Reichardt''s polarity parameters (ETN) were measured from the ultraviolet-visible spectral shifts of 4-nitroaniline, 4-nitroanisole, and Reichardt''s dye. The solvent properties of the IL-water mixtures were found to be influenced by IL type and IL mole fraction (xIL). All these studied systems showed the non-ideal behavior. The maximum deviation to ideality for the solvatochromic parameters can be obtained in the xIL range from 0.1 to 0.3. For most of the binary mixtures, the π* values showed the synergistic effects instead of the ETN, α and β values. The observed synergy extent was dependent on the studied systems, such as the dye indicator and IL type. A preferential solvation model was utilized to gather information on the molecular interactions in the mixtures. The dye indicator was preferentially solvated on the following trend: IL >IL-water complex >water. 相似文献
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