Ultraviolet B Irradiation Modulates the Immune System of Fish (Rutilus rutilus, Cyprinidae). I. Phagocytes

Roach ( Rutilus rutilus ) were irradiated with a single dose of ultraviolet B (UVB) radiation (0.4 J/cm²) in order to study the effects of UVB on the nonspecific immune defense mechanisms of fish. Neutrophils and macrophages were isolated from the head kidney of fish on days 1–14 postirradiation. Both random and directed migration of neutrophils, studied by migration under agarose assay, were suppressed on day 1 after UVB irradiation. The respiratory burst of phorbol 12-myristate 13-acetatestimulated neutrophils and macrophages was also suppressed at days 1 and 2 after UVB irradiation. The suppression of migration and respiratory burst were restored or the responses were even enhanced later, but on the other hand spontaneous cytotoxicity of neutrophils toward ⁵¹chromium-labeled K562 target cells stayed suppressed throughout the 14 day follow-up. This study indicates that UVB radiation has the potential to suppress the functioning of phagocytes and to compromise the immune system of fish.     

Ultraviolet B irradiation modulates the immune system of fish (Rutilus rutilus, Cyprinidae). II: Blood

The effects of a single dose of ultraviolet B (UVB) radiation (0.4 J/cm2) on immunological functions by blood leukocytes and on hematological parameters was studied in roach (Rutilus rutilus), a teleostean fish. The respiratory burst of phorbol 12-myristate 13-acetate stimulated whole blood phagocytes increased significantly after UVB irradiation but spontaneous cytotoxicity of blood leukocytes toward 51chromium-labeled K562 target cells was not markedly altered. Differential cell counting revealed that UVB exposure significantly increased the proportion of granulocytes and significantly decreased the proportion of lymphocytes in the peripheral blood, whereas hematocrit and the total number of white and red blood cells were unchanged. Plasma cortisol concentration increased in UVB-exposed fish. Severe handling stress caused similar, although not as potent, effects on the measured parameters of fish blood as UVB irradiation. These observations suggest that in fish UVB brings about a stress response, which may account for the observed alterations in the immune parameters and leukocyte composition of blood. Exposure of fish to strong visible light induced no alterations in immunological or hematological parameters, making it unlikely that ultraviolet radiation mediates its effects through visual perception.     

Formation of236Pu,237Pu and238Pu isotopes by3He-induced nuclear reactions on uranium

The reaction U(³He, xn)Pu was studied by irradiating enriched²³⁵U (50.3%) and enriched²³⁸U (99.7%) targets with³He ions at the Åbo Akademi cyclotron (Turku, Finland). The incident³He ion energy in the irradiations was 27.0, 24.5 and 21.9 MeV. Plutonium was separated from the thin uranium targets by ion exchange. From the ion exchange fractions obtained, plutonium was electrodeposited on steel plates for alpha and X-ray counting. The activity of²³⁷Pu in the steel plates was determined with a Ge(Li) detector and the activity of²³⁶Pu and²³⁸Pu with a Si surface barrier detector. The cross sections of the reactions and the yields of plutonium isotopes are given.     

Comparative effects of UVA and UVB irradiation on the immune system of fish

Aquatic organisms can be harmed by the current levels of solar ultraviolet radiation. We have recently shown that exposure of fish to UVB irradiation alters the functioning of the fish immune system, but the effects of UVA radiation are unknown. The present study continues this work by characterizing UVA irradiation-induced immunological changes in fish. Roach, a cyprinid fish, were exposed to a single dose of either UVA (3.6 J/cm2) or UVB (0.5 J/cm2) irradiation. Both irradiations suppressed transiently mitogen-stimulated proliferation of blood lymphocytes. UVA, but not UVB, decreased hematocrit, plasma protein, and plasma immunoglobulin levels and increased the proportions of blood cells classified as unidentified leukocytes, possibly consisting of UVA-damaged lymphocytes. UVB, but not UVA, altered the functioning of head kidney and blood phagocytes, induced granulocytosis and lymphocytopenia in the blood and increased plasma cortisol concentration. These results imply that both UVA and UVB are potent modulators of the immune defence of fish.     

Measurement of the top-quark mass in all-hadronic decays in pp collisions at CDF II

We present a measurement of the top-quark mass Mtop in the all-hadronic decay channel tt-->W+bW-b-->q1q2bq3q4b. The analysis is performed using 310 pb-1 of sqrt[s]=1.96 TeV pp[over ] collisions collected with the CDF II detector using a multijet trigger. The mass measurement is based on an event-by-event likelihood which depends on both the sample purity and the value of the top-quark mass, using 90 possible jet-to-parton assignments in the six-jet final state. The joint likelihood of 290 selected events yields a value of Mtop=177.1+/-4.9(stat)+/-4.7(syst) GeV/c2.     

Search for a very light CP-odd Higgs boson in top quark decays from pp collisions at sqrt(s)]=1.96 TeV

We present the results of a search for a very light CP-odd Higgs boson a(1)(0) originating from top quark decays t → H(±)b → W(±(*)) a(1)(0)b, and subsequently decaying into τ+ τ-. Using a data sample corresponding to an integrated luminosity of 2.7 fb(-1) collected by the CDF II detector in pp collisions at 1.96 TeV, we perform a search for events containing a lepton, three or more jets, and an additional isolated track with transverse momentum in the range 3 to 20 GeV/c. Observed events are consistent with background sources, and 95% C.L. limits are set on the branching ratio of t → H(±)b for various masses of H(±) and a(1)(0).     

Observation of the baryonic flavor-changing neutral current decay Λb(0)→Λμ+μ-

We report the first observation of the baryonic flavor-changing neutral current decay Λ(b)(0)→Λμ(+)μ(-) with 24 signal events and a statistical significance of 5.8 Gaussian standard deviations. This measurement uses a pp collisions data sample corresponding to 6.8 fb(-1) at √s = 1.96 TeV collected by the CDF II detector at the Tevatron collider. The total and differential branching ratios for Λ(b)(0)→Λμ(+)μ(-) are measured. We find B(Λ(b)(0)→Λμ(+)μ(-))=[1.73 ± 0.42(stat) ± (syst)] × 10(-6). We also report the first measurement of the differential branching ratio of B(s)(0)→?μ(+)μ(-), using 49 signal events. In addition, we report branching ratios for B(+)→K(+)μ(+)μ(-), B(0)→K(0)μ(+)μ(-), and B→K(*)(892)μ(+)μ(-) decays.     

Invariant mass distribution of jet pairs produced in association with a W boson in pp collisions at sqrt[s]=1.96 TeV

We report a study of the invariant mass distribution of jet pairs produced in association with a W boson using data collected with the CDF detector which correspond to an integrated luminosity of 4.3 fb(-1). The observed distribution has an excess in the 120-160 GeV/c(2) mass range which is not described by current theoretical predictions within the statistical and systematic uncertainties. In this Letter, we report studies of the properties of this excess.     

Search for chargino-neutralino production in pp collisions at sq rt[s] = 1.96 TeV

We present a search for associated production of the chargino and neutralino supersymmetric particles using up to 1.1 fb{-1} of integrated luminosity collected by the CDF II experiment at the Tevatron pp collider at sq rt[s]=1.96 TeV. We analyze events with a large transverse momentum imbalance and either three charged leptons or two charged leptons of the same electric charge. The numbers of observed events are consistent with standard model expectations. Upper limits on the production cross section are derived in different theoretical models.