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This paper theoretically studies the free energy and conformational entropy of a long polymer threading a long nanopore (no/N ≥0.1) on external electric field. The polymer expanded model is built in this paper, that is, a single long polymer chain with N monomers (each of size a) threading a pore with no monomers can be regarded as polymer with N + no monomers translocating a 2-dimension hole embedded in membrane. A theoretical approach is presented which explicitly takes into account the nucleation theory. Our calculations imply that, the structure of polymer changes more acutely than other situation, while its leading monomer reaches the second vacuum and its end monomer escapes the first vacuum. And it is also shown that the length scale of polymer and pore play a very important role for polymer translocation dynamics. The present model predicts that the translocation time depends on the chemical potential gradient and the property of the solvent on sides of pore to some extent. 相似文献
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Elaborate calibration procedure for cell irradiation at the CAS-LIBB single-particle microbeam 下载免费PDF全文
Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point that relates the microbeam's location to the microscope's plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam, which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup. 相似文献
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