Determination of Four Active Ingredients in Vc Yinqiao Tablets by Capillary Zone Electrophoresis with Amperometric Detection†

A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection （CZE-AD）, has been developed for simultaneous determination of four active ingredients in Vc Yinqiao tablets including paracetamol, vitamin C, caffeic acid and chlorogenic acid. A carbon-disk electrode was used as working electrode and 0.95 V （versus SCE） was selected as detection potential. The optimal conditions of CZE experiment were 30 mmol·L^-1 borate solution （pH 9.5） as running buffer, 14 kV as separation voltage and 8 s （14 kV） as electro-kinetic sampling time. Under the selected optimum conditions, paracetamol, vitamin C, caffeic acid and chlorogenic acid could be perfectly separated within 22 min, and their detection limits （S/N=3） ranged from 5×10^-7 to 1 ×10^-6 mol·L^-1. This proposed method demonstrated good reproducibility with relative standard deviations of less than 3% for both migration time and peak current （n=7）. The utility of this method was demonstrated by monitoring a kind of compound medicine named Vc Yinqiao tablets and the assay results were satisfactory.     

毛细管电泳-电化学检测法测定血浆中水溶性小分子抗氧化剂

应用毛细管电泳电化学检测方法,以金属铜电极为检测电极,研究了同时测定人血浆中水溶性小分子抗氧化剂谷胱甘肽(GSH)、尿酸(UA)、色氨酸(Try)和半胱氨酸(Cys)的最佳条件。在最佳实验条件下,各组分的线性范围在1.0×10-6～5.0×10-4mol/L内;检出限在10-6mol/L数量级。该法具有分析速度快、灵敏度高等优点,对血浆样品的测定取得了满意的结果。