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1.
Nonlinear Dynamics - A complex system’s structural–dynamical interplay plays a profound role in determining its collective behavior. Irregular behavior in the form of macroscopic chaos,... 相似文献
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Dr. Rainer Müller Dr. Mevlut Citir Dr. Sebastian Hauke Prof. Dr. Carsten Schultz 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(2):384-389
Phosphatidylinositol (PI) is the biosynthetic precursor for seven phosphoinositides, important signaling lipids in cells. A membrane-permeant caged PI derivative featuring a photo-removable coumarinyl group masking the negative charge of the phosphate, as well as two enzymatically removable butyrate esters for increased lipophilicity and for preventing phosphate migration, were synthesized. Rapid cell entry and cellular labeling in fixed cells was demonstrated by a photo-cross-linkable diazirine followed by attachment of a fluorophore through click chemistry. Using this technique, we found that the multifunctional caged PI derivative resided predominantly at internal membranes but rapidly changed to the plasma membrane after uncaging. Accordingly, a preliminary proteomic analysis of the lipid–protein conjugates revealed that the two major PI transport proteins PITPα and β were prime targets of the photo-cross-linked PI derivative. 相似文献
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Daniel Schultz 《The Ramanujan Journal》2014,34(1):119-128
A short and simple proof of the Rogers–Selberg identities is obtained from the work of Andrew Sills on identities of the Rogers–Ramanujan type. 相似文献
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Dr. Yong Zhang Dr. Huafei Zou Dr. Ying Wang Dawna Caballero Jose Gonzalez Elizabeth Chao Gus Welzel Dr. Weijun Shen Dr. Danling Wang Dr. Peter G. Schultz Dr. Feng Wang 《Angewandte Chemie (International ed. in English)》2015,54(7):2126-2130
Bovine antibody BLV1H12 possesses a unique “stalk–knob” architecture in its ultralong heavy chain CDR3, allowing substitutions of the “knob” domain with protein agonists to generate functional antibody chimeras. We have generated a humanized glucagon‐like peptide‐1 (GLP‐1) receptor agonist antibody by first introducing a coiled‐coil “stalk” into CDR3H of the antibody herceptin. Exendin‐4 (Ex‐4), a GLP‐1 receptor agonist, was then fused to the engineered stalk with flexible linkers, and a Factor Xa cleavage site was inserted immediately in front of Ex‐4 to allow release of the N‐terminus of the fused peptide. The resulting clipped herceptin–Ex‐4 fusion protein is more potent in vitro in activating GLP‐1 receptors than the Ex‐4 peptide. The clipped herceptin–Ex‐4 has an extended plasma half‐life of approximately four days and sustained control of blood glucose levels for more than a week in mice. This work provides a novel approach to the development of human or humanized agonist antibodies as therapeutics. 相似文献
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The cross-correlation between surface temperature and subsurface velocity is presented for water undergoing evaporative convection.
The surface temperature measurements were obtained via quantitative infrared imaging, and the velocity measurements were obtained
using a two-component laser Doppler velocimetry (LDV) system. The water surface was covered with a surfactant monolayer, which
did not impede evaporation. The largest measured correlation coefficient was 0.375. Correlation coefficients obtained after
shifting the velocity and temperature time traces with respect to each other revealed a peak in the correlation coefficient
very close to a zero time shift. The significance of these results and their implications for the remote sensing of underwater
objects via infrared imaging are discussed.
Received: 2 April 2001/Accepted: 30 November 2001 相似文献
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