Cell separations using targeted monoclonal antibodies against overproduced surface proteins

Applied Biochemistry and Biotechnology - The capacity of polystyrene microspheres with immobilized antibodies against type 1 pili ofE. coli was measured. Using pure IgG-type monoclonal antibodies...     

Synthesis,antioxidant, and antimicrobial activity of 3-(1H-indole-3-carbonyl)-2H-chromen-2-ones

A simple and convenient method for the one-pot synthesis of 3-(1H-indole-3-carbonyl)-2H-chromen-2-one derivatives from the reaction of 3-cyanoacetyl indole and salicylaldehyde in the presence of Na₂CO₃ in water: methanol (1:1) is described. Wider substrate scope, high yields, operational simplicity, and simple purification process make the protocol highly applicable in the synthesis of 3-(1H-indole-3-carbonyl)-2H-chromen-2-ones. For the first time, in vitro antioxidant and antimicrobial activity was studied. Compounds 5e , 7a , and 7b exhibits good radical scavenging ability against DPPH free radical. Compounds 7b , 5f , and 5g possess lower EC₅₀ values than the Standards AA and BHA and thus proving their high reducing power. Compounds 5d and 5f show good antibacterial activity against gram-positive bacteria (MRSA) while compounds 5c , 7a , and 7b exhibits good antibacterial activity against Bacillus sp. Compounds 5b and 5e show good antibacterial activity against gram negative bacterial strains (Escherichia coli, Klebsiella pneumoniae) and compounds 5g and 5h exhibits good antifungal activity against Candida albicans.     

Synthesis,characterization, thermal stability and antibacterial activity of coumarin based methacrylate copolymers

Monomer of 7-methacryloyloxy-4-methylcoumarin(MAOMC) was synthesized and characterized by FTIR, 1H-NMR and 13C-NMR spectroscopy. Copolymers of MAOMC with butoxyethylmethacrylate(BOEMA) at different feed compositions were prepared by free radical solution polymerization at(70 ± 1) °C in ethylmethylketone(EMK) using benzyl peroxide(BPO) as an initiator. The copolymers were characterized by FTIR and 1H-NMR spectroscopy. Thermogravimetric analysis(TGA) and differential scanning calorimetry(DSC) of the copolymers showed moderate thermal stability and higher Tg values. Gel permeation chromatography(GPC) was used to find out the molecular weights of the different copolymers. Antibacterial activities of the copolymers were also investigated against the selected pathogenic bacteria's. The antibacterial activity of the copolymer increases as the MAOMC content increases in the copolymer. This shows that coumarin moiety plays a very important role in the antibacterial activity.     

Synthesis of 2,3-disubstituted pyrazines and quinoxalines by Heck cross-coupling reactions of 2,3-dichloropyrazine and 2,3-dichloroquinoxaline. Influence of the temperature on the product distribution

Heck cross-coupling reactions of 2,3-dichloropyrazine provide a convenient approach to 2,3-dialkenyl-, 2-alkenyl-3-alkyl-, and 2,3-dialkylpyrazines depending on the reaction conditions.     

Brønsted acid-catalyzed simple and efficient synthesis of 1,2,4-triazoles and 1,2,4-oxadiazoles using 2,2,2-trichloroethyl imidates in PEG

A facile and highly efficient synthesis of 3,4,5-trisubstituted 1,2,4-triazoles and 3,5-disubstituted 1,2,4-oxadiazoles from 2,2,2-trichloroethyl imidates using PEG as a solvent and employing PTSA as the catalyst under mild conditions is described.     

The Internal Bremsstrahlung Accompanying the β-Decay from 185W

The internal bremsstrahlung (IB) spectrum accompanying the β-decay from ¹⁸⁵W radionuclide has been measured. The obtained spectrum was analyzed into its constituting gamma lines taking into account all the proper corrections. The analyzed spectrum was compared with those theoretically calculated. The experimental results for the IB probability were found to be in good agreement with theoretical calculations especially with the shape corrected modified KUB theory of Nilsson up to 357.7 keV.     

Expeditious,Nano-BF3 · SiO2-Catalyzed Michaelis–Arbuzov Reaction in an Ionic Liquid: Synthesis of Privileged Aryl/Heterocyclic Phosphonates

An expeditious, simple, and green method was developed for the synthesis of privileged aryl/heterocyclicphosphonates, 8(a–c) to 13(a–c) through Michaelis–Arbuzov reaction of aryl/heterocyclic halides (Br), 1–6, and trialkylphosphites, 7(a–c), in room-temperature ionic liquid [bbim]Br using heterogeneous Lewis catalyst, nano-silica-supported boron trifluoride (BF₃-SiO₂). The advantages of this protocol are simplicity, good yield of the products, less reaction time (20–38 min), mild reaction conditions, easy workup, and reusability of the catalyst and ionic liquid. It is demonstrated that nano-BF₃-SiO₂ is a recoverable and easy accessible catalyst for the formation of C(sp²)-P bond in an ionic liquid.     

Best Proximity Points: Optimal Solutions

This article elicits a best proximity point theorem for non-self-proximal contractions. As a consequence, it ascertains the existence of an optimal approximate solution to some equations for which it is plausible that there is no solution. Moreover, an algorithm is exhibited to determine such an optimal approximate solution designated as a best proximity point. It is interesting to observe that the preceding best proximity point theorem includes the famous Banach contraction principle.     

Best Proximity Point Theorems for Some Special Proximal Contractions

This article explores some new best proximity point theorems for absolute proximal cyclic contractions and dual supreme proximal contractions which are not necessarily continuous. As a consequence of such best proximity point theorems, the famous contraction principle is elicited.