Electrochemical Modulation of the Fluorescence of Cyanine Dye Cy5

Organic fluorescent dyes are widely used in single molecule localization microscopy, where their performances are determined by the photophysical properties. Herein, we utilized a sensitive method to modulate the fluorescence of organic dyes by external potentials using a combination of electrochemical cell and super‐resolution fluorescent microscopy. Cy5 (cyanine dye) was chosen as a model molecule considering its wide application and commercial availability. We applied different potentials on the Au electrode to change the Coulombic charge microenvironment of Cy5. When the electrode potential was adjusted negatively, Cy5 displayed a better photostability. This method is proved effective in adjusting the fluorescence of organic dyes.     

地质工程计算机辅助设计支持系统及其应用

地质工程设计具有非结构化、非参数化、非规范化特征,同时,也具有“风险与优化”性和“反馈与可变更”性。仅用一种固定的、程式化的求解方法是不现实的。因此,必须寻求一种非结构化的工具,根据问题的需要,面向目标自动生成求解结构。本文归纳了地质工程设计的基本内容,探讨了基于几何和基于人工智能的计算机辅助地质工程设计。最后,介绍了地质工程设计支持系统在长江三峡链子崖危岩治理工程设计中的应用。     

Atomic Imaging of Subsurface Interstitial Hydrogen and Insights into Surface Reactivity of Palladium Hydrides

Resolving interstitial hydrogen atoms at the surfaces and interfaces is crucial for understanding the mechanical and physicochemical properties of metal hydrides. Although palladium (Pd) hydrides hold important applications in hydrogen storage and electrocatalysis, the atomic position of interstitial hydrogen at Pd hydride near surfaces still remains undetermined. We report the first direct imaging of subsurface hydrogen atoms absorbed in Pd nanoparticles by using differentiated and integrated differential phase contrast within an aberration-corrected scanning transmission electron microscope. In contrast to the well-established octahedral interstitial sites for hydrogen in the bulk, subsurface hydrogen atoms are directly identified to occupy the tetrahedral interstices. DFT calculations show that the amount and the occupation type of subsurface hydrogen atoms play an indispensable role in fine-tuning the electronic structure and associated chemical reactivity of the Pd surface.     

基于光纤布拉格光栅的智能服装人体测温模型研究

根据热量传递机理建立了智能服装中光纤布拉格光栅人体测温的热传递物理模型,对人体、空气层和服装之间的热传递进行了有限元建模和稳态热分析,确定了智能服装中光纤布拉格光栅温度场的数学模型,利用该数学模型对光纤布拉格光栅测量温度值进行了修正.在多点加权皮肤平均温度的基础上,提出了由左右胸、左右腋和后背五处皮肤温度构成的智能服装...     

Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 μm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.     

The colorimetric and ratiometric fluorescent detection of cyanide and sulfide in live cells,application for logic gate and bioimging

We described the design and synthesis of a colorimetric and ratiometric fluorescent probe based on a conjugated π-electron system that displayed differential fluorescence responses towards cyanide and sulfide and demonstrated its utility in intracellular ion imaging and logic gate.     

公平的知识签名

针对一般的知识签名不能避免隐秘通信的发生这一问题,提出了知识签名的公平件概念,并给出了严格的定义.利用知识的公平的零各识证明,构造了一个公平的知识签名方案,最后证明了此签名方案可有效地保证公平性,从而避免隐秘通信的发生.     

扫描探针显微技术中脱氧核糖核酸样品的制备

评述了近年来原子力显微镜和扫描隧道显微镜应用于脱氧核糖核酸研究领域的样品制备过程.主要内容涉及基底的选择与处理、样品的固定与展开方法、优缺点以及改进过程.     

量子阱非对称法布里-珀罗腔调制器电折变模式蓝移

对于从实验中观察到的GaAs/GaAlAs多量子讲非对称法布里一哨罗腔调制器中电致折射率变化引起的模式蓝移现象,从理论上予以讨论.利用等效光程方法分析了电致折射引起的模式的移动特性,实验和理论符合较好.