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1.
Azita Fazelkhah Samaneh Afshar Nicholas Durham Michael Butler Elham Salimi Greg Bridges Douglas Thomson 《Electrophoresis》2020,41(9):720-728
In this work, we present an optical transit DEP flow cytometer for parallel single-cell analysis. Each cell's dielectric property is inferred from velocity perturbations due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two transit shadows for each cell passing through the channel. These shadows are detected using a 256-pixel linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. A wide channel (∼18 mm) was employed to carry many particles simultaneously, and the system was capable of detecting the velocity of over 200 cells simultaneously. We have achieved analysis rates for 10 µm diameter polystyrene spheres response exceeding 250 per second. With appropriate calibration, this DEP cytometer can quantitatively measure the dielectric response. The dielectric response (Clausius–Mossotti factor) of viable CHO cells was measured over the frequency range of 100 kHz to 6 MHz, and the obtained response matches the previously measured values by our group. The DEP cytometer uses simple modular components to achieve high throughput label-free single-cell dielectric analysis and can begin analyzing particles within 10 s after starting to pump the sample into the channel. 相似文献
2.
Thomas L. Theis Bhavik R. Bakshi Delcie Durham Vasilis M. Fthenakis Timothy G. Gutowski Jacqueline A. Isaacs Thomas Seager Mark R. Wiesner 《固体物理学:研究快报》2011,5(9):312-317
While significant advances in our understanding of the behavior of engineered nanoparticles in the environment continue, there remains a need to engage the nanoparticle research community directly in the development and evaluation of environmentally benign nanoparticles to ensure that nanomaterial‐based industries emerge as tools for sustainability rather than environmental liabilities. Current research efforts aimed at understanding the environmental implications of nanotechnology emphasize existing groups of nanoparticles and products already in commercial distribution. While this is clearly necessary, this approach fails to identify and address the many tradeoffs associated with product performance and environmental quality. We believe this to be a critical gap in the ongoing exploration of nanostructured materials and their properties and applications. We posit that a number of issues are not being holistically addressed, including resource availability and allocation, manufacturing energy requirements and embodied energy, material efficiency, environmental properties of nanomaterials and nanoproducts, and waste generation. An interdisciplinary approach to research, based on the life cycle paradigm and devoted to the identification, investigation, synthesis, testing, and analysis of groups of new, more environmentally conscious nanoparticles is needed. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
3.
We report a mild and efficient method for introducing deuterium into a range of heterocycles by reacting readily available halide analogues in a deuterodehalogenation reaction using D8-IPA or Et3SiD under palladium-catalysed conditions. 相似文献
4.
We present first principles KKRCPA calculations of the angle-resolved photoemission spectra from CuNi single crystal surfaces. Two surface states have been identified; a Shockley-type state on the (111) surface, and a Tamm-type state on the (100) surface. We determine their dispersion, and investigate their sensitivity to the form of the surface potential. The disorder broadening and the influence of surface segregation are discussed quantitatively. 相似文献
5.
Changes in vocal fold oscillation threshold pressure were induced in excised canine larynges by experimentally causing fluid movement into and out of the vocal folds. The transport was facilitated by exposing the vocal folds to various osmotic solutions, and it was assumed that changes in hydration caused changes in the internal tissue viscosity. A range of oscillation threshold pressures was measured for each condition of hydration by varying length and glottal width. The oscillation threshold pressure shifted as predicted. Decreased hydration (increased viscosity) raised the threshold of oscillation, and increased hydration (decreased viscosity) lowered the threshold of oscillation. This apparently represents the first in vitro model for the study of the effect of viscosity changes of the internal environment of the vocal folds on phonation. 相似文献
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Microwave reaction of RuCl3 with 2,2'-bipyridinyl-4,4'-dicarboxylic acid diethyl ester (debpy) in ethylene glycol generated Ru(bpy)3(2+) instead of the expected Ru(debpy)3(2+). Gas chromatography-mass spectrometry analysis of the headspace revealed CO2, and Ru(bpy)3(2+) was recovered from the filtrate. Further experiments suggest that RuCl3 decarboxylates debpy during microwave synthesis. 相似文献
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Opium samples from four different locations and poppy straw from different plant varieties have been assayed using micellar capillary electrophoresis incorporating a sweeping technique. Individual alkaloids (morphine, codeine, papaverine, noscapine, thebaine, oripavine, reticuline and narceine) were quantitatively determined in the different samples by a validated capillary electrophoresis method. Unsupervised pattern recognition of the opium samples and the poppy straw samples using hierarchical cluster analysis (HCA) and principal component analysis (PCA), showed distinct clusters. Supervised pattern recognition using soft independent modelling of class analogy (SIMCA) was performed to show individual groupings and allow unknown samples to be classified according to the models built using the CZE assay results. 相似文献