以变色酸2R为底物测定辣根过氧化物酶的研究

变色酸 2R(CT2R)是一种具有电化学活性的物质 ,在碱性条件下 ,该物质在滴汞电极上 - 6 6 0mV(vs.SCE)左右产生还原波 ,加入H2 O2 和辣根过氧化物酶 (HRP)后 ,该物质被氧化生成具有非电化学活性的物质 ,导致溶液中游离的CT2R浓度降低 ,相应还原波波高降低 ,HRP在一定含量范围内与伏安峰的降低值具有良好的线性关系。通过测定波高的降低值可测定HRP ,测定游离HRP的线性范围是 4 .0× 10 -5～ 4 .0× 10 -3 g/L。对酶促反应的动力学进行了研究 ,反应的米氏常数Km =1.38mmol/L ,最大反应速率Vmax=5 3.9nA/min。     

Synthesis,Structure,and Characterization of a Novel Coordination Polymer with Polythreading Feature

A novel coordination polymer 1 with formula of Zn₂(BDC)₂(TPPA) was built from 1,4-dicarboxybenzene (H₂BDC),tris(4-(pyridin-3-yl)phenyl)amine (TPPA),and Zn(Ⅱ) ion.It was characterized by single-crystal and powder X-ray diffraction,Fourier-transform infrared spectra (FT-IR),thermal gravimetric analysis (TGA),and UV-vis.1 crystallizes in triclinic space group P1 with a=14.589,b=14.606,c=16.108?,α=115.635~o,β=90.328~°,γ=114.662~o,V=2738.6A³,Z=2,M_r=935.52 g/mol,D_c=1.134 g/cm~3,F(000)=956,GOOF=1.062,the final R=0.0685 and w R=0.1817 for 7701 observed reflections with (I>2σ(I)),and R=0.0717 and w R=0.1843 for all data.In 1,Zn(Ⅱ) is coordinated with H₂BDC to form a 2D square grid,and TPPA ligands were arranged above and beneath the 2D layer.Two neighbor layers are interpenetrated each other byπ-πinteractions to generate a polythreading framework structure.Moreover,the photoluminescence of 1 was also studied.     

工业管路湍流阻力特性区域的划分

本文对 Prandtl 光滑律和 Karman 粗糙律在工业管路中的适用范围,重新作了界定,可以满足工程计算精度的要求。导出的光滑区界限判别参数,纠正了现行通用参数的错误.     

湍流管径问题算法的改进

本文采服无量纲参数（ｇＪＯ＾３／ｖ＾５）及ＫｓＶ／Ｑ）由最小二乘法以指数函数拟合Ｐｒａｎｄｔｌ及Ｋａｒｍａｎ公式，得出分别适用于光滑区和粗糙区的管径算式。再对以上两式作粘性关联偶合，得出适用于湍流过渡区的管径算式。可直接求出管径，避免繁复试算和迭代。计算结果与Ｐｒａｎｄｔｌ，Ｋａｒｍａｎ及Ｃｏｌｅｌｒｏｏｋ－Ｗｈｉｔｅ等经典公式能精确吻合，较Ｓｗａｍｅｅ－Ｊａｉｎ湍流管径算式误差减少一半以上。     

功能化PbS量子点的水相合成及结构表征

在水溶液中以Pb(NO3)2和Na2S为原料,巯基乙酸为稳定剂,合成了水溶性PbS量子点.用透射电子显微镜、扫描电子显微镜、粒度分析仪和红外光谱对PbS量子点进行了表征,结果表明所合成的PbS量子点的平均粒径为25 nm左右,分散性好,且巯基乙酸成功修饰于PbS纳米粒子表面,使其具有进一步与生物分子偶联的作用.