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蚕豆叶中具DCIP光还原活性的D_1-D_2-Cyt.b_(559)复合物的纯化和特性研究 总被引:1,自引:1,他引:1
本文首次从蚕豆叶中纯化出含有D_1,D_2和Cyt.b_(559)三种多肽的PSII反应中心复合物.该复合物在DPC存在下具有明显的光还原DCIP的活性,且在照光和暗条件下均显示Signal IIs.该复合物还含有一定数量的锰,光照下Mn~(2+)-EPR信号强度下降约40%,转入暗中Mn~(2+)-EPR信号强度又得到恢复.这些结果表明:此复合物具有从PSII第二电子供体Z到稳定原初电子受体Q_A的完整电子传递链的功能;该复合物中还可能具有锰原子结合部位,光暗转换可引起所含锰原子价态的可逆变化. 相似文献
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A PSII reaction center complex consisting of three polypeptides, D_1, D_2 and Cyt. b_(559), was first purified from broad bean leaves. The complex was fairly active inDCIP photoreduction in the presence of DPC, and showed signal Ⅱs either in the dark or under illumination. The complex also contained manganese atoms. Its Mn~(2+)-EPR intensity decreased by about 40% under continuous illumination and recovered to the original level when the complex was transferred to the dark. The above results indicated that the complex reported here contains all of the PSII electron transport chain components from the secondary donor Z to the stable primary electron acceptor Q_A, and it is possible that the complex contains manganese binding sites. The alternation in dark and illumination can induce reversible valence changes of the manganese atoms in the purified complex. 相似文献
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光解原子碘类物质包括CF3I,CH3I,C3F7I等多氟碘化物类物质,其作为激光工作介质具有增益系数大、能级寿命长等特点,适合作为大能量、高功率激光器的工作介质。这类激光器同时也具有装置结构简单、输出能量高等特点,在激光加工、诊断技术等多个领域具有广泛的应用。 相似文献
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