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"Turn-on" optical detection of parts-per-million (ppm) levels of ascorbic acid (AA) in water has been determined using a redox-active monolayer on glass. 相似文献
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A simple and ecofriendly protocol has been developed for the expedient synthesis of novel dihydroindeno[1,2-b]pyrrol-4(3aH)-ones from the reaction of ninhydrin and (E)-N-methyl-1-(methylthio)-2-nitroethenamine or (E)-N-(1-(methylthio)-2-nitrovinyl)anilines. 相似文献
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Y.L. Jeyachandran J.A. Mielczarski E. Mielczarski B. Rai 《Journal of colloid and interface science》2010,341(1):136-142
The efficiency of a pre-absorbed bovine serum albumin (BSA) layer in blocking the non-specific adsorption of different proteins on hydrophobic and hydrophilic surfaces was evaluated qualitatively and quantitatively using infrared reflection spectroscopy supported by spectral simulations. A BSA layer with a surface coverage of 35% of a close-packed monolayer exhibited a blocking efficiency of 90–100% on a hydrophobic and 68–100% on a hydrophilic surface, with respect to the non-specific adsorption of concanavalin A (Con A), immunoglobulin G (IgG), and staphylococcal protein A (SpA). This BSA layer was produced using a solution concentration of 1 mg/mL and 30 min incubation time. BSA layers that were adsorbed at conditions commonly employed for blocking (a 12 h incubation time and a solution concentration of 10 mg/mL) exhibited a blocking activity that involved competitive adsorption–desorption. This activity resulted from the formation of BSA–phosphate surface complexes, which correlated with the conformation of adsorbed BSA molecules that was favourable for blocking. The importance of optimisation of the adsorbed BSA layer for different surfaces and proteins to achieve efficient blocking was addressed in this study. 相似文献
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The 1,3-dipolar cycloaddition of azomethine ylides generated in situ from the reaction of acenaphthylene-1,2-dione or isatins and α-amino acids to (E)-methyl/ethyl 2-(3-oxo-3,4-dihydro-2H-benzo[b][1,4]thiazin-2-ylidene)acetate led to the stereoselective formation of novel dispiro 1,4-benzothiazine hybrid heterocycles in good yields. 相似文献
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Kamaraj BalamuruganVeerappan Jeyachandran Subbu Perumal J. Carlos Menéndez 《Tetrahedron》2011,67(7):1432-1437
A simple and efficient one-pot synthesis of novel 6-amino-8-aryl-2-methyl/benzyl-7-nitro-1,2,3,4-tetrahydroisoquinoline-5-carbonitriles from the reaction of 1-methyl/benzylpiperidin-4-one with β-nitrostyrenes and malononitrile in the presence of morpholine is described. This transformation proceeds through the creation of three CC bonds, leading to a new benzene ring, and presumably occurs via a domino sequence involving Knoevenagel, Michael, Thorpe-Ziegler, and dehydrogenation reactions. 相似文献
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R. P. Kengne-Momo Y. L. Jeyachandran A. Assaf C. Esnault P. Daniel J. F. Pilard M. J. Durand F. Lagarde E. Dongo G. Thouand 《Analytical and bioanalytical chemistry》2010,398(3):1249-1255
We present a new and advanced methodology, developed for surface functionalisation of gold and to study immobilisation of
an immuno-specific system of proteins. A combination of electrochemical quartz crystal microbalance and Raman spectroscopy
techniques allowed a complete understanding of the system starting from surface functionalisation and progressing to the functional
structure analysis of immobilised proteins. A simple electrochemical procedure was formulated to prepare sulphonyl chloride
terminated gold surfaces that form a strong sulphonamide bond with the receptor protein staphylococcal protein A (SpA). On
the SpA grafted surfaces, the immobilisation of a human IgG and consecutive binding of an immuno-specific anti-human IgG was
observed. The surface functional groups form a strong interaction with SpA without disturbing its functional properties. The
native functional structure of SpA and also the IgGs was found to be retained in their immobilised state. 相似文献
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