尾壳核的12.5kD蛋白-黑质DA能神经元诱向因子

本文研究新生大鼠尾壳核提取液(CPe)对黑质DA能神经元的效应。MTT微量自动比色检测含CPe培养24h,48h黑质神经元的OD值,与无CPe培养的对照组相比较,其差异分别为P<0.05和P<0.05。用预先经RITC逆行标记的黑质DA能神经元,从电泳CPe凝胶蛋白带中钓出12.5kD蛋白带,而这些神经元对DA单抗免疫组化染色呈阳性反应。含12.5kD凝胶条与黑质脑组织块近距离培养7天,显示细胞突起定向地朝12.5kD凝胶条方向生长,这类突起对DA单抗免疫组化染色呈阳性反应。这些结果表明,CPe中12.5kD蛋白分子具有维持和促进黑质DA能神经元的存活和突起定向生长的生物效应。因此将12.5kD蛋白分子命名为DA能神经元诱向因子(DNTF)。     

家兔下丘脑与腰髓间的直接联系

本文将辣根过氧化物酶(HRP)注射于7例家兔的腰6段内以后,追踪了下丘脑中的标记.除观察了逆行标记细胞的分布外,还发现了明确的顺行标记纤维及终枝. 下丘脑-脊髓投射细胞主要分布于注射侧的下丘脑后区、背侧区、外侧区及室旁核中;穹窿周围核中也有一些;少见或偶见于结节区、乳头体上核及视上核之背侧方. 脊髓-下丘脑投射主要终止于对侧,经两条入路:(1)背侧纵束,分布于下丘脑后区,可能还至乳头体上核;(2)在乳头体丘脑束的腹侧方由外向内侧横行,止于下丘脑内、外侧区的背侧部,少量散入腹侧区。     

DIRECT CONNECTIONS BETWEEN HYPOTHALAMUS AND LUMBAR SPINAL CORD IN RABBITS

Horseradish peroxidase (HRP) was injected into the L6 spinal cord in seven rabbits andthe labeling in the hypothalamus traced. The distribution of the retrogradely labeled cellswas studied and the anterogradely labeled fibers and terminal branches were unequivocallyidentified. Hypothalamo-spinal projection cells were found predominantly on the ipsilateral side,mainly distributed in the posterior, dorsal, and lateral hypothalamic areas, and the paraven-tricular nucleus. Some were found in the perifornical nuelcus and a few could be seen in thetuber area, supranmmnfillary nucleus and tho region doisal to the supraoptic nucleus. Spino-hypothalamic projections were found to terminate mainly on the contralateral sidevia two. routes: (i) The dorsal longitudinal fascieulus, distributed to the posterior hypotha-lamie area. Some might end in the supramammillary nucleus; (ii) Fibers running latero-medially ventral to the mammillothalamic tract to terminate in the dorsal parts of the medialand lateral hypothalanms