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1.
miRNA (miR)-155 is a potential biomarker for breast cancers. We aimed at developing a nanosensor for miR-155 detection by integrating hybridization chain reaction (HCR) and silver nanoclusters (AgNCs). HCR serves as an enzyme-free and isothermal amplification method, whereas AgNCs provide a built-in fluorogenic detection probe that could simplify the downstream analysis. The two components were integrated by adding a nucleation sequence of AgNCs to the hairpin of HCR. The working principle was based on the influence of microenvironment towards the hosted AgNCs, whereby unfolding of hairpin upon HCR has manipulated the distance between the hosted AgNCs and cytosine-rich toehold region of hairpin. As such, the dominant emission of AgNCs changed from red to yellow in the absence and presence of miR-155, enabling a ratiometric measurement of miR with high sensitivity. The limit of detection (LOD) of our HCR-AgNCs nanosensor is 1.13 fM in buffered solution. We have also tested the assay in diluted serum samples, with comparable LOD of 1.58 fM obtained. This shows the great promise of our HCR-AgNCs nanosensor for clinical application. 相似文献
2.
Bao Nguyen Xuan Duy Khanh Phan Quoc Tung Nguyen Minh 《Set-Valued and Variational Analysis》2022,30(2):465-486
Set-Valued and Variational Analysis - We aim to establish Karush-Kuhn-Tucker multiplier rules involving higher-order complementarity slackness under Hölder metric subregularity. These rules... 相似文献
3.
A novel technique to overcome the long-term drift and hysteresis of a scanning Fabry–Perot filter was developed and applied to wavelength and power monitoring of DWDM system. By using the comb peaks generated by a temperature-stabilized, near threshold-biased Fabry–Perot diode laser as wavelength reference for the scanning Fabry–Perot filter, wavelength and power measurement accuracy of better than ±10 pm and 0.2 dB, respectively, were achieved. 相似文献
4.
5.
Ching Sum Hui Lillian Sze Man Lam Chun Yin Wai Kin Chan Aleksandra B. Djurii 《Journal of polymer science. Part A, Polymer chemistry》2003,41(11):1708-1715
Polyurethanes incorporated with rhenium diimine complexes were synthesized. The polymers exhibited interesting morphologies and solution properties. Results from gel permeation chromatography suggested the formation of polymer aggregates in solutions. The polymers could act as probes for metal ions. The addition of metal ions to polymer solutions led to significant changes in the electronic absorption properties of the polymer solutions. This was attributed to the interactions between the polyether moieties and metal ions. The metal complexes could also act as efficient photosensitizers. After doping with charge‐transport viologens, the photoconductivity of the polymers was greatly enhanced. The experimental quantum efficiency was simulated with Onsager's theory. The thermalization distances and the primary yields were typically 12–14 Å and 10?3, respectively. © 2003 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 41: 1708–1715, 2003 相似文献
6.
The natural modes of a discrete linear system are orthogonal with respect to the mass and stiffness matrices in a generalized sense. However, these modes are usually not orthogonal to each other in the ordinary sense. The purpose of this paper is to document a number of conditions under which the modes are also orthogonal in an ordinary fashion. 相似文献
7.
Siaw-Lynn Ng 《Order》2004,21(1):1-5
We present a characterisation of posets of size n with linear discrepancy n − 2. These are the posets that are “furthest” from a linear order without being an antichain.
This revised version was published online in September 2006 with corrections to the Cover Date. 相似文献
8.
We will prove a result concerning the inclusion of non-trivial invariant ideals inside non-trivial ideals of a twisted crossed
product. We will also give results concerning the primeness and simplicity of crossed products of twisted actions of locally
compact groups on -algebras.
Received: 25 January 2002; in final form: 22 May 2002/Published online: 2 December 2002
This work is partially supported by Hong Kong RGC Direct Grant. 相似文献
9.
A boundary element method for the calculation of noise barrier insertion loss in the presence of atmospheric turbulence 总被引:1,自引:0,他引:1
Yiu Wai Lam 《Applied Acoustics》2004,65(6):583-603
Atmospheric turbulence is an important factor that limits the amount of attenuation a barrier can provide in the outdoor environment. It is therefore important to develop a reliable method to predict its effect on barrier performance. The boundary element method (BEM) has been shown to be a very effective technique for predicting barrier insertion loss in the absence of turbulence. This paper develops a simple and efficient modification of the BEM formulation to predict the insertion loss of a barrier in the presence of atmospheric turbulence. The modification is based on two alternative methods: (1) random realisations of log-amplitude and phase fluctuations of boundary sources and (2) de-correlation of source coherence using the mutual coherence function (MCF). An investigation into the behaviours of these two methods is carried out and simplified forms of the methods developed. Some systematic differences between the predictions from the methods are found. When incorporated into the BEM formulation, the method of random realisations and the method of MCF de-correlation provide predictions that agree well with predictions by the parabolic equation method and by the scattering cross-section method on a variety of thin barrier configurations. 相似文献
10.
Discrete global descent method for discrete global optimization and nonlinear integer programming 总被引:2,自引:0,他引:2
A novel method, entitled the discrete global descent method, is developed in this paper to solve discrete global optimization
problems and nonlinear integer programming problems. This method moves from one discrete minimizer of the objective function
f to another better one at each iteration with the help of an auxiliary function, entitled the discrete global descent function.
The discrete global descent function guarantees that its discrete minimizers coincide with the better discrete minimizers
of f under some standard assumptions. This property also ensures that a better discrete minimizer of f can be found by some classical local search methods. Numerical experiments on several test problems with up to 100 integer
variables and up to 1.38 × 10104 feasible points have demonstrated the applicability and efficiency of the proposed method. 相似文献