INVESTIGATION OF THE ANTIVIRAL ACTION OF THE PHOTOACTIVE COMPOUND PHENYLHEPTATRIYNE

Abstract— Murine cytomegalovirus, a herpes virus, was used as a model virus to investigate the mechanism of the anti-viral action of phenylheptatriyne in long wave ultraviolet light. The genome and proteins of the inactivated virus penetrated the nuclei of susceptible cells normally. Furthermore, the viral genome did not contain extra single strand breaks or cross-links. However, cells infected with the treated virus did not synthesize late viral proteins, as determined by polyacrylamide gel electrophoresis. nor did they synthesize late viral RNA and viral DNA according to nucleic acid hybridization tests. Thus the compound may interfere with an early viral function so that the replication cycle cannot proceed.     

STRUCTURE-ACTIVITY STUDIES OF PHOTOACTIVATED ANTIVIRAL AND CYTOTOXIC TRICYCLIC THIOPHENES

The photoactivated antiviral and cytotoxic activities of the naturally occurring thiophene, alpha-terthienyl (1), and 15 synthetic analogues were evaluated against murine cytomegalovirus and Sindbis virus, and murine mastocytoma cells. After irradiation with near UV light, alpha-terthienyl and most of its analogues had significant toxicity, with minimum inhibitory concentrations in the range of 0.02-40 microM. In the absence of near UV irradiation, only one analogue had antiviral activity and five were cytotoxic. The most active analogues were those containing carboxylic acid, hydroxyl, or cyano substituents. Quantitative structure-activity relationship analysis of thiophene phototoxicity suggested that the rate of singlet oxygen production is the primary determinant of antiviral and cytotoxic activities. For phototoxicity against murine cytomegalovirus, a significant role for hydrophobicity was also demonstrated. Tricyclic thiophenes show significant potential for photochemotherapy of viral infections and cancer, and further evaluation in animal models is recommended.     

Generalized frequency selection in multifrequency interferometry

We present a generalized frequency selection method for N-frequency interferometry to form an optimum geometric series at synthetic wavelengths. The absolute range that is measurable is bounded by the number of beat frequency operations, phase noise, and the number of wavelengths used to form the geometric series of synthetic wavelengths. Theoretical predictions are compared with experimental results from a full-field fringe projector. A comparison of this technique with the method of excess fractions shows orders-of-magnitude faster processing with similar measurement reliability.     

A measurement of the proton structure functions from neutrino-hydrogen and antineutrino-hydrogen charged-current interactions

Within the framework of the quark-parton model, the quark and anti-quark structure functions of the proton have been measured by fitting them to the distributions of the events in the Bjorkeny variable. The data used form the largest sample of neutrino and antineutrino interactions on a pure hydrogen target available, and come from exposures of BEBC to the CERN wide band neutrino and antineutrino beams. It is found that the ratiod _v /u _v of valence quark distributions falls with increasing Bjorkenx. In the context of the quark-parton model the results constrain the isospin composition of the accompanying diquark system. Models involving scattering from diquarks are in disagreement with the data.     

On abelian subalgebras and ideals of maximal dimension in supersolvable Lie algebras

In this paper, the main objective is to compare the abelian subalgebras and ideals of maximal dimension for finite-dimensional supersolvable Lie algebras. We characterise the maximal abelian subalgebras of solvable Lie algebras and study solvable Lie algebras containing an abelian subalgebra of codimension 2. Finally, we prove that nilpotent Lie algebras with an abelian subalgebra of codimension 3 contain an abelian ideal with the same dimension, provided that the characteristic of the underlying field is not 2. Throughout the paper, we also give several examples to clarify some results.     

Strain and vibration analysis by fibre based speckle shearing interferometry

An optical fibre based, speckle shearing interferometer is described. The instrument uses a highly birefringent optical fibre to illuminate a test object with equal intensities of light guided by the orthogonal polarisation eigenstates of the fibre. A Wollaston prism is used to obtain two sheared images with adjustable shear. Optical phase changes between the sheared images are readily achieved, without mechanical movement of components, by straining the optical fibre. Object strain determination, by fringe analysis with phase stepping techniques, is readily achieved. Vibration analysis by heterodyning is also reported.     

Wavefront sensing for single view three-component three-dimensional flow velocimetry

We present the application of wavefront sensing to particle image velocimetry for three-component (3C), three-dimensional (3D) flow measurement from a single view. The technique is based upon measuring the wavefront scattered by a tracer particle and from that wavefront the 3D tracer location can be determined. Hence, from a temporally resolved sequence of 3D particle locations the velocity vector field is obtained. Two approaches to capture the data required to measure the wavefronts are described: multi-planar imaging using a distorted diffraction grating and an anamorphic technique. Both techniques are optically efficient, robust and compatible with coherent and incoherent scattering from flow tracers. The depth (range) resolution and repeatability have been quantified experimentally using a single mode fiber source representing a tracer particle. The anamorphic approach is shown to have the greatest measurement range and hence was selected for the first proof of principle experiments using this technique for 3D particle imaging velocimetry (PIV) on a sparsely seeded gas phase flow.     

抗坏血酸和尿酸在甘氨酸-壳聚糖修饰玻碳电极上的电化学行为及测定

制备了甘氨酸-壳聚糖复合膜修饰玻碳电极(Gly-CTS/GCE),研究了抗坏血酸(AA)和尿酸(UA)在该修饰电极上的电化学行为。结果表明在pH=5.59的磷酸盐缓冲溶液中,AA、UA在Gly-CTS/GCE上均产生灵敏的不可逆氧化峰,其峰电流与浓度在一定范围内呈良好的线性关系。对AA和UA混合溶液平行测定7次,相对标准偏差分别为4.6%、2.9%,表明该电极重现性和稳定性良好。AA、UA在Gly-CTS/GCE电极上的氧化峰峰电位相差340mV,据此可实现对二者的同时检测,并可应用于实际样品测定。